Publications by authors named "L Farrokh-Siar"
Trypan blue induces apoptosis in human retinal pigment epithelial cells.
Am J Ophthalmol
September 2004
Purpose: To examine whether trypan blue dye induces apoptosis in human retinal pigment epithelium cells.
Design: Laboratory investigation.
Methods: Pure cultures of human retinal pigment epithelium cells were isolated.
Purpose: To examine whether indocyanine green (ICG) dye induces apoptosis in human retinal pigment epithelial (RPE) cells.
Design: Laboratory investigation.
Methods: Pure cultures of human RPE cells were isolated.
Purpose: To examine the regulatory effects of interferon (IFN)-alpha, IFN-gamma, transforming growth factor (TGF)-beta, and tumor necrosis factor (TNF)-alpha on human fetal retinal pigment epithelial (HFRPE) cell-induced apoptosis of human Jurkat T (Jkt) cells.
Methods: Pure cultures of HFRPE cells were isolated. The cells were precultured with medium alone or with addition of IFN-alpha, IFN-gamma, TNF-alpha, or TGF-beta for 72 hours.
Purpose: To evaluate whether human fetal retinal pigment epithelial (HFRPE) cells express TRAIL (tumor necrosis factor related apoptosis inducing ligand). The role of TRAIL in HFRPE induced apoptosis was evaluated.
Methods: Pure cultures of HFRPE cells were isolated.
Purpose: Experiments were performed to evaluate the effect of various pro- and anti-inflammatory cytokines on the human fetal retinal pigment epithelium's (HFRPE) expression of major histocompatibility complex (MHC) and costimulatory molecules.
Methods: Pure cultures of HFRPE cells were isolated. HFRPE cells were incubated in the presence of Interferon-gamma (IFN-gamma), IFN-beta, Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-1beta (IL-1beta), Tumor Growth Factor-beta (TGF-beta), and a combination of IFN-gamma and TGF-beta (pre-incubation and simultaneously incubated).