Identification of novel Kv1.3 channel-interacting proteins using proximity labelling in T-cells.

Potassium channels regulate membrane potential, calcium flux, cellular activation and effector functions of adaptive and innate immune cells. The voltage-activated Kv1.3 channel is an important regulator of T cell-mediated autoimmunity and microglia-mediated neuroinflammation.

ESG introduction mechanism of construction firms based on Bayesian network coupled with machine learning: Evidence from Zhengzhou.

Chinese construction enterprises are at a pivotal point in their transition to sustainable development, with Environmental, Social, and Governance (ESG) emerging as a key driver. However, limited understanding of ESG mechanisms hampers effective management strategies. To address this challenge, this study constructs an ESG introduction mechanism framework based on Bayesian networks and machine learning algorithms.

Adolescents Constructing Their Imagined Futures Through Dialogues With and/or About Nonfamilial Adults.

Introduction: Adolescents actively explore future imaginings as they prepare for major educational, work, and personal transitions. Although family members may support exploration of imagined futures, adults outside adolescents' kin network provide access to resources not supplied within the family. The purpose of this study was to understand how adolescents actively draw on social resources of nonfamilial adults relative to their imagined futures.

Quantifying UV-induced photodamage for longitudinal live-cell imaging applications of deep-UV microscopy.

Deep-UV microscopy enables high-resolution, label-free molecular imaging by leveraging biomolecular absorption properties in the UV spectrum. Recent advances in UV-imaging hardware have renewed interest in this technique for quantitative live cell imaging applications. However, UV-induced photodamage remains a concern for longitudinal dynamic imaging studies.

β3 accelerates microtubule plus end maturation through a divergent lateral interface.

β-tubulin isotypes exhibit similar sequences but different activities, suggesting that limited sequence divergence is functionally important. We investigated this hypothesis for TUBB3/β3, a β-tubulin linked to aggressive cancers and chemoresistance in humans. We created mutant yeast strains with β-tubulin alleles that mimic variant residues in β3 and find that residues at the lateral interface are sufficient to alter microtubule dynamics and response to microtubule targeting agents.