Publications by authors named "L F R Gebert"
Small interfering RNAs (siRNAs) guide mRNA cleavage by human Argonaute2 (hAgo2), leading to targeted gene silencing. Despite their laboratory and clinical impact, structural insights into human siRNA catalytic activity remain elusive. Here, we show that disrupting siRNA 3'-end binding by hAgo2 accelerates target cleavage and stabilizes its catalytic conformation, enabling detailed structural analysis.
View Article and Find Full Text PDFCo-transcriptional assembly is an integral feature of the formation of RNA-protein complexes that mediate translation. For ribosome synthesis, prior studies have indicated that the strict order of transcription of rRNA domains may not be obligatory during bacterial ribosome biogenesis, since a series of circularly permuted rRNAs are viable. In this work, we report the structural insights into assembly of the bacterial ribosome large subunit (LSU) based on cryo-EM density maps of intermediates that accumulate during in vitro ribosome synthesis using a set of circularly permuted (CiPer) rRNAs.
View Article and Find Full Text PDFCo-transcriptional assembly is an integral feature of the formation of RNA-protein complexes that mediate translation. For ribosome synthesis, prior studies have indicated that the strict order of transcription of rRNA domains may not be obligatory during bacterial ribosome biogenesis, since a series of circularly permuted rRNAs are viable. In this work, we report the insights into assembly of the bacterial ribosome large subunit (LSU) based on cryo-EM density maps of intermediates that accumulate during ribosome synthesis using a set of circularly permuted (CiPer) rRNAs.
View Article and Find Full Text PDF[Complications Following Treatment for Excessive Axillary Sweating With Microwaves: A Case Report].
Ann Burns Fire Disasters
December 2022
Excessive axillary sweating is a frequent reason for seeking consultation in aesthetic medicine. Botulic toxin therapies have been used for years for this condition. A microwave-based treatment (MiraDry) has been used in France since 2011.
View Article and Find Full Text PDFArticle Synopsis
- Patients with inborn errors of immunity (IEI) experience higher morbidity and mortality from COVID-19, with age, comorbidities, and impaired type I interferon immunity identified as key risk factors.
- A study on five patients with primary antibody deficiency (PAD) who faced severe COVID-19 showed that, despite lacking a specific humoral immune response, they developed a strong T cell response and exhibited signs of an activated memory-like response.
- Results indicated prolonged viral shedding, with some patients maintaining SARS-CoV-2 RNA in their blood even after treatment, suggesting the need for closer monitoring and potential treatments like convalescent plasma for severe cases lacking antibody response.