Publications by authors named "L F Minnullina"
We studied the effect of urinary urea concentration on the hemolysin production and cytotoxicity of the uropathogenic Morganella morganii strain MM 190. The highest hemolytic activity of M. morganii cultivated in urine with low urea concentration (23 and 82 mmol/liter) was observed between 3rd and 4th hours of post-inoculation, while in urine with standard urea level (117 mmol/liter), the activity was observed at 5th hour of post-inoculation.
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- The study investigated how different growth media and aeration affect the hemolytic activity of uropathogenic Morganella morganii strain MM 190.
- The highest hemolysis levels were recorded in LB (59%), DMEM with fetal bovine serum (62%), and urine (53%) during the exponential growth phase, while 2% urea hindered hemolysin production.
- The hemolysin gene hlyA peaked in expression after 2 hours in LB medium, aligning with the observed hemolytic activity, indicating that the short hlyCA transcript is dominant in these conditions.
In current times, the opportunistic pathogen Morganella morganii is increasingly becoming a cause of urinary tract infections. The condition has been further complicated by the multiple drug resistance of most isolates. Swimming motility plays an important role in the development of urinary tract infections, allowing bacteria to colonize the upper urinary tract.
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- The study focused on sequencing and analyzing the genomes of three strains of an opportunistic bacterial pathogen from patients with urinary tract infections.
- The whole genome sizes ranged from 3.82 to 3.97 Mb, with a high percentage of protein-coding sequences and various genomic features identified, including prophages and genomic islands.
- Notably, specific genes linked to virulence, such as those for urease, hemolysis, and adhesion proteins, were found, with some strains showing significant cytotoxic effects on bladder cancer cells.