A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories.

Setting up a global SARS-CoV-2 surveillance system requires an understanding of how virus isolation and propagation practices, use of animal or human sera, and different neutralisation assay platforms influence assessment of SARS-CoV-2 antigenicity. In this study, with the contribution of 15 independent laboratories across all WHO regions, we carried out a controlled analysis of neutralisation assay platforms using the first WHO International Standard for antibodies to SARS-CoV-2 variants of concern (source: NIBSC). Live virus isolates (source: WHO BioHub or individual labs) or spike plasmids (individual labs) for pseudovirus production were used to perform neutralisation assays using the same serum panels.

Behavioral, haematological, and physiological effects of oxygen-rich spray after simulated catch and release in rainbow trout (Oncorhynchus mykiss).

This study investigated the impact of an oxygen-rich spray on rainbow trout subjected to short-term air exposure episodes. These episodes can be due to sampling procedures or catch-and-release (C&R) practices, focusing on behavioral, haematological, and physiological responses. For this, 12 rainbow trout were divided into two groups: control and oxygen-rich sprayed fish.

Enhancing farmed fish welfare: Evaluating the effectiveness of plant-based stress mitigating agents as sedatives in sea bass (Dicentrarchus labrax) following intraperitoneal vaccination.

The effectiveness of vaccines may be compromised by the stress response induced by intraperitoneal/intramuscular (IP/IM) vaccination due to an intimate interaction between the neuroendocrine and immune systems. Essential oils (EOs), known for their antibacterial, antioxidant, and sedative properties, are potential candidates to mitigate this stress response. This study investigates the short-term sedative effect of two essential oil-based products, FishEase-C (FEC) and FishEase-L (FEL) in sea bass prior to intraperitoneal vaccination (IP).

Sea water acclimation of rainbow trout (Oncorhynchus mykiss) modulates the mucosal transcript immune response induced by Vibrio anguillarum and Aeromonas salmonicida vaccine, and prevents further transcription of stress-immune genes in response to acute stress.

Mucosal tissues appear to be more important in fish than in mammals due to living in a microbial-rich aquatic milieu, yet the complex interaction between the immune and the neuroendocrine system in these tissues remains elusive. The aim of this work was to investigate the mucosal immune response in immunized rainbow trout vaccinated with Alpha ject vaccine (bivalent), kept in fresh water (FW) or transferred to seawater (SW), and to evaluate their response to acute stress (chasing). Acute stress resulted in higher levels of plasma cortisol (Sham + Stress and Vaccine + Stress).