Enhanced subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach.

In 2006, a PCR method was introduced to subtype by Sanger sequencing of an ≈610 bp amplicon of the 18S rRNA gene. This method, known as barcoding-PCR, has become widespread, although the primer pair used can amplify non- sequences, which can result in false positives. Barcoding-PCR is most effective with DNA extracted from cultures, limiting its sensitivity when used directly with stool samples.

The impact of the Covid-19 pandemic on work accidents with exposure to biological material in Brazil: an interrupted time series analysis.

Objective: To analyze the impact of the Covid-19 pandemic on the occurrence of work accidents involving biological materials (ATBio) and to assess changes in the epidemiological profile of these cases.

Methods: An ecological time-series study with a cross-sectional component was conducted using ATBio notifications in Brazil from 2015 to 2022 in the Information System for Notifiable Diseases (Sinan). Interrupted time-series analyses were performed using Prais-Winsten regression models, temporal predictions, and multiple logistic regression to identify changes in the profile between the pandemic and pre-pandemic periods.

Characterisation of the tumour microenvironment and PD-L1 granularity reveals the prognostic value of cancer-associated myofibroblasts in non-invasive bladder cancer.

High-risk non-muscle-invasive bladder cancer (NMIBC) presents high recurrence and progression rates. Despite the use of Bacillus Calmette-Guérin gold-standard immunotherapy and the recent irruption of anti-PD-1/PD-L1 drugs, we are missing a comprehensive understanding of the tumor microenvironment (TME) that may help us find biomarkers associated to treatment outcome. Here, we prospectively analyzed TME composition and PD-L1 expression of tumor and non-tumoral tissue biopsies from 73 NMIBC patients and used scRNA-seq, transcriptomic cohorts and tissue micro-array to validate the prognostic value of cell types of interest.

3DPatBody: 3D dataset of human bodies of a patagonian population and their anthropometric measurements.

The study of human body shape using classical anthropometric techniques is often problematic due to several error sources. Instead, 3D models and representations provide more accurate registrations, which are stable across acquisitions, and enable more precise, systematic, and fast measuring capabilities. Thus, the same person can be scanned several times and precise differential measurements can be established in an accurate manner.

Data on vascular plant species composition along two elevation gradients in the high Andes of central Chile (33°S).

The results of a survey of vascular plant species along two elevational gradients in the high Andes of central Chile (33°S) are reported. Vascular plant species were recorded in 12 and 15 plots of 1600 m set up to be permanent, distributed at intervals of 100 m elevation along two elevational gradients, one in the La Parva (2500-3600 m a.s.