Publications by authors named "L Estrada-Capetillo"
Background And Aims: GM-CSF-dependent macrophage polarization has been demonstrated in rheumatoid arthritis (RA). Our aim was to seek diagnostic/prognostic biomarkers for undifferentiated arthritis (UA) by analyzing GM-CSF expression and source, macrophage polarization and density in joints of patients with UA evolving to RA or PsA compared with established RA or PsA, respectively.
Methods: Synovial tissue (ST) from patients with UA evolving to RA (UA>RA, n=8), PsA (UA>PsA, n=9), persistent UA (UA, n=16), established RA (n=12) and PsA (n=10), and healthy controls (n=6), were analyzed.
CD28 expression is generally considered to be T lymphocyte specific. We have previously shown CD28 mRNA expression in M-CSF-dependent anti-inflammatory monocyte-derived macrophages (M-MØ), and now demonstrate that CD28 cell surface expression is higher in M-MØ than in GM-CSF-dependent macrophages, and that macrophage CD28 expression is regulated by MAFB and activin A. In vivo, CD28 was found in tumor-associated macrophages and, to a lower extent, in pro-inflammatory synovial fluid macrophages from rheumatoid arthritis patients.
View Article and Find Full Text PDFThis study tested the hypothesis that vasoactive intestinal peptide (VIP) is able to modify the macrophage inflammatory profile, thus supporting its therapeutic role in autoimmune diseases. Macrophages are innate immune cells that display a variety of functions and inflammatory profiles in response to the environment that critically controls their polarization. Deregulation between the pro- and anti-inflammatory phenotypes has been involved in different pathologies.
View Article and Find Full Text PDFArticle Synopsis
- Methotrexate (MTX) has distinct effects on macrophages involved in rheumatoid arthritis (RA), identified through its transcriptional influence on proinflammatory (GM-MØ) and anti-inflammatory (M-MØ) macrophage subtypes.
- Research methods included gene expression profiling and molecular pathway analysis to understand how MTX affects these macrophages.
- The study found that MTX primarily alters gene expression in proinflammatory GM-MØ, revealing that their sensitivity to MTX is linked to the expression of thymidylate synthase (TS) and the activity of p53, with CCL20 and LIF identified as potential markers for this responsiveness.
Background & Aims: Patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. We aimed at evaluating Bifidobacterium pseudocatenulatum CECT7765 effect on the host's macrophage function.
Patients & Methods: Patients with cirrhosis and ascites were included.