Some vasovasostomized men are characterized by low levels of P34H, an epididymal sperm protein.

During epididymal transit, sperm surface proteins involved in the fertilization process can be added or modified. P34H, a human epididymal-sperm protein, is proposed to be involved in the interactions between spermatozoa and the zona pellucida. We have previously demonstrated that P34H is present in men of proven fertility and is absent in 50% of men presenting with idiopathic infertility.

Human endometrial cells cultured in a type I collagen gel.

Objective: To elaborate in vitro conditions that enable epithelial and stromal cells of human endometrium to grow within a gel of collagen.

Study Design: Primary cultures of epithelial cells derived from human endometrial biopsies were dissociated and mixed with a collagen solution, and the gel was allowed to form at physiologic pH. Control cultures were grown in plastic dishes.