Construction and Validation of CRISPR/Cas Vectors for Editing the Gene in Banana ( spp.).

Bananas and plantains are important staple food crops affected by biotic and abiotic stresses. The gene editing technique via Clustered Regularly Interspaced Short Palindromic Repeats associated with the Cas protein (CRISPR/Cas) has been used as an important tool for development of cultivars with high tolerance to stresses. This study sought to develop a protocol for the construction of vectors for gene knockout.

Aging promotes an increase in mitochondrial fragmentation in astrocytes.

Introduction: Brain aging involves a complex interplay of cellular and molecular changes, including metabolic alterations and the accumulation of senescent cells. These changes frequently manifest as dysregulation in glucose metabolism and mitochondrial function, leading to reduced energy production, increased oxidative stress, and mitochondrial dysfunction-key contributors to age-related neurodegenerative diseases.

Methods: We conducted experiments on two models: young (3-4 months) and aged (over 18 months) mice, as well as cultures of senescent and control mouse astrocytes.

Demographic profile of surgical approaches to obstructive sleep apnea in the United States from 2004 to 2020: a descriptive study.

Objectives: This study aims to evaluate the quantity, types, and trends of surgical procedures used to treat obstructive sleep apnea (OSA) within a diverse national population, utilizing a comprehensive proprietary healthcare database.

Methods: This descriptive observational study analyzed longitudinal data from the Optum Clinformatics® Data Mart databases, covering the period from January 2004 to December 2020. The study included patients aged 18 to 89 years, both male and female, with a confirmed diagnosis of OSA.

Susceptibility of Brazilian populations of Rachiplusia nu (Lepidoptera: Noctuidae) to insecticides and determination of diagnostic concentrations for a proactive resistance management program.

Background: The sunflower looper, Rachiplusia nu (Guenée), evolved resistance to the insecticidal protein Cry1Ac expressed in soybean and emerged as an important soybean pest in Brazil, requiring the application of insecticides for their control. Here, we characterized the susceptibility of Brazilian populations of R. nu to several insecticides and developed diagnostic concentrations for a proactive insect resistance management (IRM) program.

Anti-Inflammatory and Antioxidant Activities of Eugenol: An Update.

Medicinal plants are a rich source of bioactive compounds that possess pharmacological properties for preventing and treating inflammation-related diseases. Essential oils is a chemical class that contains many bioactive compounds, such as eugenol, which is capable of inhibiting or modulating the inflammatory response. This natural product emerges as a compound that promotes various biological activities, including antioxidant activity, which makes it useful in the food industry.