Simplifying the protocol for low-pollution-risk, efficient mouse myoblast isolation and differentiation.

Myoblasts are the primary effector cells that play crucial roles in myogenesis and muscle regeneration following injury. However, isolating purified primary myoblasts from murine skeletal muscle poses challenges for junior researchers. Here, we present a simplified, low-risk, and optimized protocol for the extraction and enrichment of these myogenic progenitor cells.

Research on the mechanical properties and micro damage mechanism of water bearing sandstone under uniaxial compression.

To provide a theoretical basis for the development of water hazard prevention schemes for roof sandstone, this study systematically conducts uniaxial compression, acoustic emission, scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), and X-ray diffraction (XRD) tests on the thick water-bearing sandstone at depths of 623-943 m in the Luohe Formation of the Binchang Mining Area, investigating its mechanical properties and the mechanisms of micro-damage evolution under uniaxial compression.The results indicate that with the increase in water content, the elastic modulus, strength, peak strain, and brittleness index of sandstone decrease. Dry sandstone primarily undergoes shear failure, while water-saturated sandstone mainly experiences a combination of tensile and shear failure.

Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis.

Interactions between metastatic cancer cells and the brain microenvironment regulate brain metastasis (BrMet) progression. Central nervous system (CNS)-native and peripheral immune cells influence the BrMet immune landscape, but the dynamics and factors modulating this microenvironment remain unclear. As the gut microbiome impacts CNS and peripheral immune activity, we investigated its role in regulating immune response dynamics throughout BrMet stages.

Pan-cancer multi-omic model of LINE-1 activity reveals locus heterogeneity of retrotransposition efficiency.

Somatic mobilization of LINE-1 (L1) has been implicated in cancer etiology. We analyzed a recent TCGA data release comprised of nearly 5000 pan-cancer paired tumor-normal whole-genome sequencing (WGS) samples and ~9000 tumor RNA samples. We developed TotalReCall an improved algorithm and pipeline for detection of L1 retrotransposition (RT), finding high correlation between L1 expression and "RT burden" per sample.

Endometriosis: A new perspective on epigenetics and oxidative stress.

As a complex chronic gynecological disorder characterized by multifaceted etiology involving genetics, environment, immunity and inflammation, endometriosis (EM) has long been a significant concern for women of reproductive age worldwide. This review aimed to comprehensively examine the interplay between epigenetics and oxidative stress (OS) in the pathogenesis of EM. Through the integration of cutting-edge research, the response of OS signals to epigenetic modifications was explored.