Exploring the expression of Pseudomonas aeruginosa genes directly from sputa of cystic fibrosis patients.

Unlabelled: Acquisition of Pseudomonas aeruginosa is known as a negative prognostic factor in patients with cystic fibrosis. We started a pilot study to evaluate Ps. aeruginosa gene expression directly from the sputum of infected patients.

Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.

Practice of noninvasive ventilation for cystic fibrosis: a nationwide survey in France.

Background: No guidelines are available for noninvasive ventilation (NIV) for cystic fibrosis (CF).

Objective: To survey and evaluate the use of NIV for CF in France.

Methods: We surveyed the coordinator physicians of every accredited CF center in France.