Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.

For the past fifteen years, significant research advances in sequencing technology have led to a substantial increase in fruit tree genomic resources and databases with a massive number of OMICS datasets (transcriptomic, proteomics, metabolomics), helping to find associations between gene(s) and performance traits. Meanwhile, new technology tools have emerged for gain- and loss-of-function studies, specifically in gene silencing and developing tractable plant models for genetic transformation. Additionally, innovative and adapted transformation protocols have optimized genetic engineering in most fruit trees.

Impact of hormone applications on ripening-related metabolites in Gewürztraminer grapes (Vitis vinifera L.): The key role of jasmonates in terpene modulation.

Terpenes play a formative role in grape and wine flavor, particularly for high-terpenic cultivars. Differences in terpene profiles influence grape varietal character and vintage quality. Little is known about the endogenous factors controlling terpene biosynthesis in grape.

Gas Chromatography Coupled to Atmospheric Pressure Chemical Ionization High-Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L) Berry.

This chapter describes the application of atmospheric pressure chemical ionization in conjunction with gas chromatography (APGC) coupled to high-resolution mass spectrometry for profiling metabolites in plant and fruit extracts. The APGC technique yields molecular ions and limited fragmentation of volatile or derivatized compounds. The data-independent acquisition mode, MS, was used for measuring precursor and fragment ions with high resolution using a quadrupole ion mobility time-of-flight mass spectrometry system.

The role of auxin during early berry development in grapevine as revealed by transcript profiling from pollination to fruit set.

Auxin is a key phytohormone that modulates fruit formation in many fleshy fruits through the regulation of cell division and expansion. Auxin content rapidly increases after pollination and the manipulation in its levels may lead to the parthenocarpic development. ln Vitis vinifera L.

Nitrate Uptake and Transport Properties of Two Grapevine Rootstocks With Varying Vigor.

In viticulture, rootstocks are essential to cope with edaphic constraints. They can also be used to modulate scion growth and development to help improve berry yield and quality. The rootstock contribution to scion growth is not fully understood.