Enhancing evidence-based practice in radiography education: Evaluation of an applied curriculum intervention.

Introduction: It is imperative that radiography graduates are taught to effectively apply evidence-based practice (EBP) in their clinical careers. Teaching the concept is challenging, as it requires educators to embed and scaffold knowledge and skills across the EBP cycle throughout both academic and clinical curricula. The objective of this study was to evaluate an overarching curriculum level intervention that aimed to enhance student radiographers' knowledge, skills, attitudes and practices towards EBP.

Enzyme-Responsive DNA Condensates.

Membrane-less compartments and organelles are widely acknowledged for their role in regulating cellular processes, and there is an urgent need to harness their full potential as both structural and functional elements of synthetic cells. Despite rapid progress, synthetically recapitulating the nonequilibrium, spatially distributed responses of natural membrane-less organelles remains elusive. Here, we demonstrate that the activity of nucleic-acid cleaving enzymes can be localized within DNA-based membrane-less compartments by sequestering the respective DNA or RNA substrates.

Innovative Learning Activities to Prepare Radiography Students for Final-Year Clinical Placements: An Educational Perspective.

Introduction/background: The aim of this educational perspective is to describe a program of learning activities developed to prepare radiography students for final year clinical placements. Authors' reflections are integrated with student feedback to discuss the educational value and feasibility of implementing the activities presented.

Methods: A program of six components was developed: (1) Simulation-based learning, (2) clinical supervision, (3) tutorials and workshops, (4) medical imaging facility learning tours (5) lecture series and (6) journal club.

Reduced Non-Specific Binding of Super-Resolution DNA-PAINT Markers by Shielded DNA-PAINT Labeling Protocols.

The DNA-based single molecule super-resolution imaging approach, DNA-PAINT, can achieve nanometer resolution of single targets. However, the approach can suffer from significant non-specific background signals originating from non-specifically bound DNA-conjugated DNA-PAINT secondary antibodies as shown here. Using dye-modified oligonucleotides the location of DNA-PAINT secondary antibody probes can easily be observed with widefield imaging prior to beginning a super-resolution measurement.

Co-transcriptional production of programmable RNA condensates and synthetic organelles.

Condensation of RNA and proteins is central to cellular functions, and the ability to program it would be valuable in synthetic biology and synthetic cell science. Here we introduce a modular platform for engineering synthetic RNA condensates from tailor-made, branched RNA nanostructures that fold and assemble co-transcriptionally. Up to three orthogonal condensates can form simultaneously and selectively accumulate fluorophores through embedded fluorescent light-up aptamers.