Publications by authors named "L Dahlgren"
Biofilms reduce antibiotic efficacy and lead to complications and mortality in human and equine patients with orthopedic infections. Equine bone marrow-derived mesenchymal stromal cells (MSC) kill planktonic bacteria and prevent biofilm formation, but their ability to disrupt established orthopedic biofilms is unknown. Our objective was to evaluate the ability of MSC to reduce established S.
View Article and Find Full Text PDFObjective: The aim of the present study was to determine if a three-dimensional (3D)-printed instrument technique would improve lavage removal of plastic beads (guttural pouch [GP] chondroid mimics) through a dorsal pharyngeal recess (DPR) fenestration. We hypothesized that using a 3D-printed instrument placed through the DPR fenestration would remove more beads, reduce lavage time and incur less soft tissue damage than using a lavage tube control or instrument placement through the salpingopharyngeal ostium (SPO).
Study Design: Experimental cadaveric study.
Osteoarthritis (OA) can be debilitating and is related to impaired resolution of synovial inflammation. Current treatments offer temporary relief of clinical signs, but have potentially deleterious side effects. Bone marrow mononuclear cells (BMNC) are a rich source of macrophage progenitors that have the ability to reduce OA symptoms in people and inflammation in experimentally-induced synovitis in horses.
View Article and Find Full Text PDFArticle Synopsis
- Osteoarthritis (OA) is linked to the inability of synovial macrophages to effectively reduce joint inflammation, and increasing macrophage presence through bone marrow mononuclear cell (BMNC) injections promotes lasting inflammation resolution.
- The study investigates how BMNC influence resolution by analyzing gene expression in response to normal and inflamed synovial fluid, revealing that BMNC initially activate pro-inflammatory pathways before enhancing anti-inflammatory networks associated with resolution.
- Key pathways identified include the mevalonate pathway and PPAR-γ signaling, which improve mitochondrial metabolism and inhibit NF-κB signaling, highlighting the complex interplay of pro- and anti-inflammatory responses necessary for effective inflammation resolution in OA.
Osteoarthritis (OA) is the most prevalent joint disease causing major disability and medical expenditures. Synovitis is a central feature of OA and is primarily driven by macrophages. Synovial macrophages not only drive inflammation but also its resolution, through a coordinated, simultaneous expression of pro- and anti-inflammatory mechanisms that are essential to counteract damage and recover homeostasis.
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