[Determination of antibodies in blood serum of patients with pulmonary tuberculosis using mycobacterial antigen affinity isolation].

Possible use of radio- and enzyme immunoassays (RIA and EIA, respectively) in serodiagnosis of tuberculosis with antigen BCG isolated by affinity was studied in comparison to the use of the routine antigen PPD. The specific IgG antibodies were determined in serum specimens of 150 patients with various forms of pulmonary tuberculosis and 50 healthy donors. A statistically reliable difference in the efficiency of tuberculosis serodiagnosis (high levels of specificity and sensitivity) by the RIA and EIA with using antigen BCG isolated by affinity was observed.

[Antitubercular antibodies to antigens of various molecular weights in patients with active pulmonary tuberculosis].

Antibodies were determined in 72 tuberculous patients and 27 healthy donors using enzyme immunoassay (EIA) and immunoblotting (IB). It was shown that the levels of the antibodies to BCG and H37Rv sonicates were lower in the patients with disseminated tuberculosis than in the patients with fibrocavernous or infiltrative tuberculosis. Significant differences in the antibody spectra in the patients with different forms of tuberculosis were also shown.