Use of confocal laser scanning microscopy to evaluate the metal ion removal and destabilization of Enterococcus faecalis biofilms by EDTA and etidronic acid.

Chelating substances bind to metals, forming stable complexes, rendering these essential ions unavailable for microbial metabolism and community stability in biofilms. This action can contribute to the disinfection in endodontic treatments. Through confocal laser scanning microscopy (CLSM), this study quantified the metal ion removal by chelating agents in Enterococcus faecalis biofilms and assessed the impact on community stability.

The Gerotranscendence Leading to Optimal Well-Being [GLOW] Program: A Feasibility Study in a Long-Term Care Facility.

Objectives: Gerotranscendence has gained recognition as an important psychosocial theory, with leading studies promoting it through intervention programs. However, to date, few interventions have involved older adults living in Long-Term Care (LTC) facilities. This study aimed to explore the viability of implementing six-week program on gerotranscendence [GLOW] in an LTC facility.

A computational approach for MHC-restricted multi-epitope vaccine design targeting Oropouche virus structural proteins.

In recent years, Brazil has recorded approximately 500,000 Oropouche virus (OROV) cases in the Amazon region, underscoring the growing global threat posed by emerging and reemerging viruses. Symptoms of OROV closely resemble those of Dengue virus and Zika virus, contributing to underreporting and underestimation of its true impact. In the absence of specific treatments, the development of vaccines becomes essential.

MORPHOLOGIC DEVELOPMENT OF AN UNIDENTIFIED SPECIES OF SARCOCYSTIS ASSOCIATED WITH ENCEPHALITIS IN A GRAY FOX (UROCYON CINEREOARGENTEUS).

Morphologic development of unidentified Sarcocystis sp. schizonts associated with encephalitis in a 9-wk-old gray fox (Urocyon cinereoargenteus) from Texas is described. Sarcocystis schizonts were confined to lesions.

Correlation between REDOX enzymes and NET markers expression during the development of periapical lesions in mice.

Objective: To evaluate and correlate the expression of REDOX enzymes and NET markers during the development of periapical lesions (PLs) in mice.

Methods: PLs were experimentally induced by pulpal exposure of first molars. In all, 42 mice were involved in a negative control and five periods (after 2, 5, 7, 21 and 42 days) (n = 7 in each period).