Publications by authors named "L Choisnard"
Article Synopsis
- Bacterial infectious diseases pose a significant public health challenge, especially with rising antibiotic resistance, making fast detection of pathogens essential for control measures.
- Current methods rely on culture techniques, which are time-consuming and require special equipment, leading to a need for alternative detection methods that can quickly identify whole bacteria without culture.
- Aptamer-based assays are emerging as a promising alternative, offering benefits such as stability, specificity, and cost-effectiveness, with various detection techniques like optical and electrochemical methods enhancing their utility in diagnosing bacterial infections.
Cardiovascular diseases, including fatal myocardial infarctions from atheromatous plaques, are the primary global mortality cause. Detecting stenotic atheromatous plaques is possible through coronary angiography, but vulnerable plaques with eccentric remodeling are undetectable with current diagnostic methods. Addressing this challenge, our group developed a radiopharmaceutical drug targeting vascular cell adhesion molecule 1 (VCAM-1), radiolabeled with technetium-99m.
View Article and Find Full Text PDFObjectives: To assess the impact of disparities in production and analytical control processes on the quality of parenteral nutrition (PN) preparations produced in the Auvergne-Rhône-Alpes region.
Methods: This study was carried out in four hospital pharmacies of the Auvergne-Rhône-Alpes region. To assess the impact of production processes, each centre produced ten PN preparations from the same prescription.
Background: The aim of this brief communication is to highlight the potential bacteriological risk linked to the processes control of radiopharmaceutical preparations made in a radiopharmacy laboratory. Survival rate of Pseudomonas aeruginosa (ATCC: 27853) or Staphylococcus aureus (ATCC: 25923) or Staphylococcus epidermidis (ATCC: 1228) in multidose technetium-99 m solution was studied.
Results: Depending on the nature and level of contamination by pathogenic bacteria, the lethal effect of radioactivity is not systematically observed.
Background: Gla-domainless factor (F) Xa (GD-FXa) was proposed as a trap to endogenous anticoagulant tissue factor pathway inhibitor (TFPI) to restore thrombin generation in hemophilia. Using computational chemistry and experimental approaches, we previously showed that S195A GD-FXa also binds TFPI and restores coagulation in plasma obtained from person(s) with hemophilia.
Methods: To design a GD-FXa variant with improved anti-TFPI affinity, we performed molecular dynamics simulations and identified suitable sites for mutagenesis.