Publications by authors named "L Carioti"

Article Synopsis
  • Next-generation sequencing (NGS) is replacing Sanger sequencing for HIV genotypic drug resistance testing, and this study assessed how consistent different interpretation tools are in a real-world setting.
  • The research involved analyzing NGS results from 11 Italian labs using the AD4SEQ HIV-1 Solution v2 kit and compared interpretations from SmartVir, HyDRA Web, and Stanford HIVdb.
  • Findings indicated that viremia levels and HIV subtypes influence the validity of NGS-GRT, with only mutations present at frequencies over 10% showing reliable agreement among the interpretation tools.
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Background: To clarify whether next-generation sequencing (NGS) can be useful for resistance assessment in virologically suppressed highly treatment-experienced (HTE) individuals with MDR HIV.

Methods: Ninety-one participants from the PRESTIGIO Registry were included. NGS was performed on HIV-DNA at 1%, 5% and 20% cut-offs; major drug resistance mutations (DRMs) were evaluated and compared with those detected in historical plasma genotypic resistance testing (h-GRT).

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Article Synopsis
  • Equine hepacivirus (EqHV) is an RNA virus linked to mild hepatitis in horses and is present worldwide, but Italy lacks comprehensive data on its prevalence.
  • A study involving 1,801 horse serum samples across various categories and regions in Italy found a national prevalence of 4.27%, with no significant differences among categories or regions.
  • The research identified three known EqHV subtypes in Italy and suggested a potential fourth subtype, highlighting the need for EqHV to be included in hepatitis diagnostic protocols and blood product safety measures.
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In this study, we provided a retrospective overview in order to better define SARS-CoV-2 variants circulating in Italy during the first two years of the pandemic, by characterizing the spike mutational profiles and their association with viral load (expressed as ct values), N-glycosylation pattern, hospitalization and vaccination. Next-generation sequencing (NGS) data were obtained from 607 individuals (among them, 298 vaccinated and/or 199 hospitalized). Different rates of hospitalization were observed over time and among variants of concern (VOCs), both in the overall population and in vaccinated individuals (Alpha: 40.

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(1) Background: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) is a promising therapeutic target to fight COVID-19, and many RdRp inhibitors nucleotide/nucleoside analogs, such as remdesivir, have been identified or are in clinical studies. However, the appearance of resistant mutations could reduce their efficacy. In the present work, we structurally evaluated the impact of RdRp mutations found at baseline in 39 patients treated with remdesivir and associated with a different degree of antiviral response in vivo.

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