Study on the Antioxidant and Anticancer Activities of Flavonoids and Its Screen of Small Molecule Active Components.

Firstly, the antioxidant capacity of total flavonoids that was extracted from was determined by ABTS+·radical-scavenging method, and MTT assay was used to mensurate the anti-tumor activity of total flavonoids In Vitro. Subsequently, the extraction conditions of flavonoids were optimized by the response surface methodology, the antioxidant activity was investigated simultaneously. Furthermore, the anti-tumor activity mechanism of flavonoids was simulated by molecular docking software.

Aberrant rhythmic expression of regulates the radiosensitivity of rat gliomas.

In this study, we investigated the role of the clock regulatory protein cryptochrome 2 (Cry2) in determining the radiosensitivity of C6 glioma cells in a rat model. We observed that Cry2 mRNA and protein levels showed aberrant rhythmic periodicity of 8 h in glioma tissues, compared to 24 h in normal brain tissue. Cry2 mRNA and protein levels did not respond to irradiation in normal tissues, but both were increased at the ZT4 (low Cry2) and ZT8 (high Cry2) time points in gliomas.