The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer.

Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, and explicitly quantifies intratumoral heterogeneity.

Early Diagnosis of Papillary Carcinoma in a Thyroglossal Duct Cyst: A Multidisciplinary Approach and the Crucial Role of Fine-Needle Aspiration Cytology.

Papillary carcinoma within a thyroglossal duct cyst is a rare and challenging presentation of thyroid malignancy, requiring a nuanced approach to diagnosis and treatment. This report details the case of a 40-year-old male who presented with a two-year history of submental swelling and intermittent respiratory difficulty. Initial imaging suggested a simple thyroglossal duct cyst.

Isolation and Characterization of Plant-Growth-Promoting Bacteria Associated with Aublet.

Aublet is a floating aquatic plant, capable of absorbing the excess of nutrients and water contaminants and can be used in effluent treatment plants. The ability to survive in degraded areas may be related to the association with beneficial bacteria capable of promoting plant growth. However, little is known about the microbiota associated with this aquatic plant and its potential application to the aquatic environment.