[Preparation of enzymes from raw materials of animal origin for medical application].

The paper outlines the basic principles of production of enzymes for medical application from animal raw material. It presents complex scheme of the enzyme preparation from the pancreas of cattle and pigs, inhibitors of proteolytic enzymes from the pancreas and lungs of cattle as well as hyaluronidase action from the testes of cattle. The paper describes preparation scheme of purification of enzymes and protease inhibitors and results of their clinical study.

[Cell cultures obtained using collalytin from the pancreases of cattle fetuses].

The authors present morphological characteristics of primary monolayer cultures prepared from the pancreas of bovine fetuses. Combined treatment with trypsin and collalytine solutions (a preparation with collagenase activity) was used for dispersion of the tissue of the pancreas. Numerous epithelial cells corresponding by morphofunctional characteristics to beta-cells of the islets of Langerhans were contained in be cultures obtained; an aldehyde-fuchsin-positive granularity was revealed in the cytoplasm of these cells.