[Sizes of bacterial cells in soils determined by cascade filtration technique].

This paper studies the number of bacteria in typical chernozem and mountain-meadow soil by the traditional method and the cascade filtration technique. The total number of bacteria in these soils, which was obtained in filters of different diameters during filtering the suspension of a certain amount, is 1.5-5 times higher than that obtained by the traditional method.

[Fungal to bacterial biomass ratio in the forest soil profile].

The carbon content in microbial biomass (Cmic-BM) was determined in various horizons of the soil profile (sod-podzo, gray, podzol, and rzhavozem) of various forests (oak, spruce Yellow Archangel, spruce moss, aspen, spruce broadleaf) in the southern taiga of European Russia (Moscow and Kaluga oblasts) by the substrate induced respiration (SIR) and direct microscopy (DM) methods. The fungi-to-bacteria ratio was measured by the selective inhibition technique and DM. A quantitative differentiation of the fungal mycelium was suggested.

[Estimation of abundance dynamics of gram-negative bacteria in soil].

Bacterial succession in soil was studied for two variants of initiation (moistening and moistening with addition of glucose). To determine the numbers of viable gram-negative bacteria, the modified nalidixic acid method was applied. The numbers of gram-negative bacteria revealed by this method were 2 to 3.

[Comparative assessment of soil microbial biomass determined by the methods of direct microscopy and substrate-induced respiration].

The content of microbial biomass (MB) was determined in samples of gray forest, chestnut, and tundra soils with different physicochemical properties (0.4-22.7% Corg; 8.

[Succession of chitinolytic microorganisms in chernozem soil].

The chitinolytic prokaryotic and eukaryotic microbial complex of chernozem soil has been investigated in the course of a succession initiated by the introduction of chitin and humidification. The dynamics of the cell numbers of chitinolytic microorganisms and of their biomass was assessed by fluorescent microscopy and by inoculation of selective media. Emission of carbon dioxide and nitrous oxide, as well as dinitrogen fixation, was assessed by gas chromatography.