Development of a biomonitoring assay for ortho-toluidine or its metabolites in human urine.

We describe a method for the measurement of a metabolite of ortho-toluidine in urine. The method uses capillary gas chromatographic separation with mass spectrometric detection to quantitate the metabolite, and it requires no derivatization or extraction of the urine sample prior to analysis. Quantitation is accomplished by comparison with a control spiked with a standard of the metabolite.