Enterobacterales carrying chromosomal AmpC β-lactamases in Europe (EuESCPM): Epidemiology and antimicrobial resistance burden from a cohort of 27 hospitals, 2020-2022.

Introduction: The ESCPM group (Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia species and Morganella morganii) has not yet been incorporated into systematic surveillance programs.

Methods: We conducted a multicentre retrospective observational study analysing all ESCPM strains isolated from blood cultures in 27 European hospitals over a 3-year period (2020-2022). Diagnostic approach, epidemiology, and antimicrobial susceptibility were investigated.

Evaluating the drop test method in measuring colistin susceptibility of , and .

Colistin is an antibiotic used to treat Gram-negative bacterial infections, particularly those caused by multidrug-resistant bacteria. The broth microdilution (BMD) reference method is the recommended protocol for detecting colistin susceptibility; however, it is laborious and expensive and cannot be performed in all laboratories. To evaluate the colistin susceptibility in , and using an alternative method, which has been referred to as the drop test.

Small-molecule activation of OGG1 increases oxidative DNA damage repair by gaining a new function.

Oxidative DNA damage is recognized by 8-oxoguanine (8-oxoG) DNA glycosylase 1 (OGG1), which excises 8-oxoG, leaving a substrate for apurinic endonuclease 1 (APE1) and initiating repair. Here, we describe a small molecule (TH10785) that interacts with the phenylalanine-319 and glycine-42 amino acids of OGG1, increases the enzyme activity 10-fold, and generates a previously undescribed β,δ-lyase enzymatic function. TH10785 controls the catalytic activity mediated by a nitrogen base within its molecular structure.

Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR.

Introduction: Enteric viruses have been associated with the production of a variety of diseases transmitted by the fecal-oral route and carried through contaminated food and water. Given their structure and composition, they are highly resistant to environmental conditions and most of the chemical agents used in the purification processes. Therefore, the systematic monitoring of raw water is necessary to ensure its quality especially when it is used for producing drinking water for human consumption.

Plurality in understandings of innovation, sociotechnical progress and sustainable development: An analysis of OECD expert narratives.

Deterministic theory and discourse on sociotechnical progress ignore the existence of multiple and equally viable pathways towards progress, obscure socioeconomic and environmental conflicting interests and values, and overshadow socially inclusive deliberative choices about policy strategies. Demystifying techno-determinism, by incorporating a plurality of understandings to policy appraisal, becomes a matter of not only democratic accountability but also of analytical rigour. This article analyses the normative and ontological understandings on scientific and technological pathways among a group of experts interviewed at one key Directorate of the Organisation for Economic Cooperation and Development, using Q-methodology.