Phytate in plants (inositol phosphates, InsPs) affects mineral bioavailability. However, methods for their quantification may lead to variable results, and some are nonspecific (spectrophotometric techniques). In this study, ion-pair high-performance liquid chromatography (HPLC) was coupled with post-column derivatization to allow fluorescence detection (FLD, λ324/λ364 nm) of InsPs.
View Article and Find Full Text PDFCooking-induced conversion of starch, the major carbohydrate in pulses, is crucial for the digestibility of the seed. The gelatinization-melting transition of lentil, bean and chickpea starches was studied using Differential Scanning Calorimetry at different temperatures (T values ranged from 20 to 160 °C) and water contents (X from 0.2 to 3 kg kg db).
View Article and Find Full Text PDFA cytohistological investigation was performed to better understand the structural alterations of cowpea seeds in relation with soaking. Thin sections obtained from seeds soaked at 30 °C, 60 °C and 95 °C were treated to specifically visualize starch, proteins, cellulose and pectin. Micropyle behavior as well as water uptake and dry matter loss were also monitored.
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