[FEATURES OF tRNA GENES AND YOUNG SUBFAMILIES Alu-REPEATS EXPRESSION IN PROLIFERATION AND APOPTOSIS].

Using human U937 cell culture, we studied the expression of the RNA polymerase III-directeci genes--tRNAi(Met)1, tRNA(His) and Alu-repeats belonging to the youngest subfamilies AluY (AluYa5 and AluYb8) - du- ring camptothecin-induced apoptosis. The level of tRNAiMetl increased 1.5-fold, tRNAHis level did not change, and the level of AluY-RNA increased 4-10-fold after 6 hours of CAM treatment compared to control (non-apoptotic) cells.

Methylation levels of SLC23A2 and NCOR2 genes correlate with spinal muscular atrophy severity.

Spinal muscular atrophy (SMA) is a monogenic neurodegenerative disorder subdivided into four different types. Whole genome methylation analysis revealed 40 CpG sites associated with genes that are significantly differentially methylated between SMA patients and healthy individuals of the same age. To investigate the contribution of methylation changes to SMA severity, we compared the methylation level of found CpG sites, designed as "targets", as well as the nearest CpG sites in regulatory regions of ARHGAP22, CDK2AP1, CHML, NCOR2, SLC23A2 and RPL9 in three groups of SMA patients.

Genome-wide analysis shows association of epigenetic changes in regulators of Rab and Rho GTPases with spinal muscular atrophy severity.

Spinal muscular atrophy (SMA) is a monogenic disorder that is subdivided into four different types and caused by survival motor neuron gene 1 (SMN1) deletion. Discordant cases of SMA suggest that there exist additional severity modifying factors, apart from the SMN2 gene copy number. Here we performed the first genome-wide methylation profiling of SMA patients and healthy individuals to study the association of DNA methylation status with the severity of the SMA phenotype.

[Effect of synthetic cyclopentane beta,beta'-triketones on amino acid metabolism in roots of buckwheat (Fagopyrum esculentum Moench.) seedlings].

Germination of buckwheat seeds in solutions of synthetic mono- and tricyclic cyclopentane-containing beta,beta'-triketones of various concentrations was accompanied by inhibition of seedling root growth and changes in the contents of glutamate, gamma-aminobutyric acid, proline, glutamine, and alanine. The monocyclic triketone also affected the amount of isoleucine. It is likely that the increase in proline content is a nonspecific response significant for enhancing stress tolerance in seedlings.