Divergent Synthesis of 3-(Indol-2-yl)quinoxalin-2-ones and 4-(Benzimidazol-2-yl)-3-methyl(aryl)cinnolines via Polyphosphoric Acid (PPA)-Mediated Intramolecular Rearrangements of 3-(Methyl/aryl(2-phenylhydrazono)methyl)quinoxalin-2-ones.

Herein, we report a polyphosphoric acid (PPA)-mediated divergent metal-free operation to access a diverse collection of 3-(indol-2-yl)quinoxalin-2-ones and 4-(benzimidazol-2-yl)-3-methylcinnolines in moderate to excellent overall yields. The described process involves two distinct, and competing rearrangements of 3-(methyl(2-phenylhydrazono)methyl)quinoxalin-2-ones, namely [3,3]-sigmatropic Fischer rearrangement with the formation of an indole ring to produce 3-(indol-2-yl)-quinoxalin-2-ones, and Mamedov rearrangement with simultaneous construction of benzimidazole and cinnoline rings to form the new biheterocyclic system─4-(benzimidazol-2-yl)-3-methylcinnolines. The reaction mechanism of both rearrangement channels is explored by extensive dispersion-corrected DFT calculations.

Bromine-Promoted One-Pot Furo[]annulation and α-C(sp)-Thiomethylation Cascade of ()-3-Styrylquinoxalin-2(1)-ones with Dimethyl Sulfoxide.

A new process has been developed for the bromine-promoted sequential (sp)C = (sp)C bond functionalization of ()-3-styrylquinoxalin-2(1)-ones and furo[]annulation via the 5-cyclization in dimethyl sulfoxide (DMSO). The reaction represents a novel strategy for the synthesis of 2-aryl-3-(methylthio)furo[2,3-]quinoxalines and involves 3-(1,2-dibromo-2-arylethyl)quinoxalin-2(1)-ones and 2-arylfuro[2,3-]quinoxalines as key intermediates. Furthermore, DMSO was converted to dimethyl sulfide in situ, which served as the methylthiolation reagent in the reaction.

[Modified method of inoculation of hemocultures from patients with suppurative-septic diseases and typhoid-paratyphoid fever].

A modified method of isolating hemocultures in pyoseptic processes is proposed. It consists in combined use of two known methods: inoculation of the blood by in-depth method and preliminary hemolysis of the blood, and use of commercial medium for assessing the sterility as the nutrient base. High efficacy of the proposed method has been demonstrated in experiments with 17 reference strains of microorganisms.