The Role of Platelet Homeostasis in a Novel Topical PRP Formulation.

Background: Topical platelet-rich plasma (PRP) must demonstrate stability to insure biologic activity in aesthetic medicine.

Objective: The objective of this research was to evaluate the role of platelet homeostasis in a novel PRP topical cosmetic formulation to provide facial appearance improvement.

Methods: The stability of the topical PRP formulation was evaluated in vitro followed by clinical in vivo testing.

An evaluation of electrocoagulation and thermal diffusion following radiofrequency microneedling using an in vivo porcine skin model.

Background: Few studies exist that examined the role of radiofrequency microneedling (RFMN) in skin electrocoagulation. This research utilized a porcine model to understand bipolar dermal delivery from an RFMN device.

Aims: The objective of this study was to elucidate and compare the dermal thermal effects of a RFMN device producing 1 and 2 MHz signal amplitudes, with respective voltage and current gradients, utilizing noninsulated and insulated needles by examining the histologic effects on porcine skin.

Pilot study: Autologous platelet-rich plasma used in a topical cream for facial rejuvenation.

Background: Platelet rich plasma (PRP) is traditionally used as an injectable material for enhanced healing, hair growth, and facial rejuvenation.

Aims: This research examined the novel use of topical autologously sourced PRP added to a preservative cosmetic base and applied twice daily to the face following electroporation for 8 weeks.

Methods: 20 healthy female and male subjects 30-60 years of age were enrolled in this single-site, investigator blinded, vehicle controlled split-face study to evaluate the effect of a PRP-containing serum versus the serum alone on facial photoaging.

Topically delivered dissolved oxygen reduces inflammation and positively influences structural proteins in healthy intact human skin.

Background: As oxygen is essential for wound healing and there is limited diffusion across the stratum corneum into the epidermis, we wanted to evaluate whether the topical delivery of a total dissolved oxygen in dressing form on intact human subject skin would improve clinical and histologic skin functioning.

Aims: Fifty normal, healthy subjects completed a pilot clinical evaluation to assess the efficacy and tolerability of a dissolved oxygen dressing (OxygeneSys™-Continuous) to improve the health and appearance of intact skin.

Methods: Clinical analysis was performed on 50 subjects; histological and gene expression analysis was performed on 12 of the 50 subjects to assess the effect of the dissolved oxygen dressing.

Human cell-conditioned media produced under embryonic-like conditions result in improved healing time after laser resurfacing.

Background: Laser resurfacing procedures are continuing to grow in popularity as patients select less invasive procedures for rejuvenation of photo-damaged and aging skin. However, although physicians have begun exploring options to aid in postlaser healing, currently available treatments have little clinical evidence to support their use for wounded skin.

Methods: When grown under conditions of very low oxygen and suspension, a simulation of the embryonic environment, neonatal cells have been found to produce proteins and growth factors in types and quantities similar to those of fetal cells.