PanKB: An interactive microbial pangenome knowledgebase for research, biotechnological innovation, and knowledge mining.

The exponential growth of microbial genome data presents unprecedented opportunities for unlocking the potential of microorganisms. The burgeoning field of pangenomics offers a framework for extracting insights from this big biological data. Recent advances in microbial pangenomic research have generated substantial data and literature, yielding valuable knowledge across diverse microbial species.

Massively targeted evaluation of therapeutic CRISPR off-targets in cells.

Methods for sensitive and high-throughput evaluation of CRISPR RNA-guided nucleases (RGNs) off-targets (OTs) are essential for advancing RGN-based gene therapies. Here we report SURRO-seq for simultaneously evaluating thousands of therapeutic RGN OTs in cells. SURRO-seq captures RGN-induced indels in cells by pooled lentiviral OTs libraries and deep sequencing, an approach comparable and complementary to OTs detection by T7 endonuclease 1, GUIDE-seq, and CIRCLE-seq.

[Changes in mackerel (Scomber scombrus, L.) skeletal muscle tissue during prolonged low-temperature preservation].

Freezing slightly changes ultrastructure of myofibrillar proteins, but is accompanied with their decreased solubility. During frozen storage, progressive decrease in solubility of these proteins by the 4th month is probably depended on the fact that in sarcoplasm certain globular particle associates appear; they are products of the most easily hydrolized proteins in filaments, namely, Z-lines, I-discs, H-zone. The fact that some protein components of the sarcomere disappear simultaneously with decreasing solubility of the myofibrillar proteins demonstrates that these processes are closely connected with each other.