Publications by authors named "L A Mirny"
Every cell must solve the problem of how to fold its genome. We describe how the folded state of chromosomes is the result of the combined activity of multiple conserved mechanisms. Homotypic affinity-driven interactions lead to spatial partitioning of active and inactive loci.
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- The cohesin protein complex helps organize chromosomes into specific domains but its biological importance is unclear.
- This study highlights that cohesin is essential for the differentiation and function of dendritic cells, particularly in antigen presentation and IL-12 secretion.
- It also shows that the interplay between cohesin and the transcription factor IRF8 influences chromatin structure, gene expression, and the activation of dendritic cells.
As cells exit mitosis and enter G1, mitotic chromosomes decompact and transcription is reestablished. Previously, Hi-C studies showed that essentially all interphase 3D genome features including A/B-compartments, TADs, and CTCF loops, are lost during mitosis. However, Hi-C remains insensitive to features such as microcompartments, nested focal interactions between -regulatory elements (CREs).
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- - S.J. Gould and R. Lewontin's "Spandrels paper" (1979) argues that some anatomical features evolve not for their function but due to other developmental and physical reasons.
- - The authors suggest that similar principles apply to molecular processes in the cell nucleus, where detectable structures may not have specific functions.
- - These nuclear structures may simply result from the processes that created them, reflecting constraints and forces rather than serving a defined purpose.
The hallmarks of chromosome organization in multicellular eukaryotes are chromosome territories (CT), chromatin compartments, and insulated domains, including topologically associated domains (TADs). Yet, most of these elements of chromosome organization are derived from analyses of a limited set of model organisms, while large eukaryotic groups, including insects, remain mostly unexplored. Here we combine Hi-C, biophysical modeling, and microscopy to characterize the 3D genome architecture of the silkmoth, .
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