Determination of cesium, manganese and terbium by intracavity laser spectroscopy.

The statistical characteristics of the intracavity laser spectrometer are investigated by analysis of cesium in aqueous solutions. The error distribution of measurements is normal. The relative standard deviation of the analysis in the middle of the dynamic range is equal to 9.

[Modification of the ligand load and structure of human serum albumin with different methods of isolation].

The influence of isolation procedures on physico-chemical properties of human serum albumin (HSA) has been studied using differential scanning calorimetry and infrared spectroscopy. Fractionation of blood plasma with polyethylene glycol (PEG) followed by ion-exchange chromatography produced healthy donor albumin whose melting thermograms were identical to those of the HSA in non-fractionated plasma. The endotherms of HSA samples isolated by affinity chromatography (AC) and native electrophoresis (EP) are bimodal as distinct from monophasic endotherms of PEG-HSA preparations.

[Automated method for separate measurement of coproporphyrins and uroporphyrins in the urine].

The authors suggest a new improved automated method for separate measurements of urinary porphyrins (coproporphyrins and uroporphyrins) in native urine with computer processing of spectral data. The method of dividing the total spectrum of porphyrin fluorescence takes account of background non-porphyrin admixtures in the urine and corrects the data on the basis of regression analysis. The data of this method were compared to those of other widely used methods and were found to be in good correlation; the advantages of the automated method are discussed.

[Quantitative gas chromatographic determination of 3-methylhistidine in biological fluids].

The gas chromatographic procedure is suggested to determine 3-methylhistidine in biological fluids. The amino acid fraction containing 3-methylhistidine is separated by ion-exchange chromatography. Amino acids are transformed into N-trifluoroacetyl-O-isobutyl esters which are analyzed by the gas chromatography instrument with micropacked columns and ionization-resonance detector.