Publications by authors named "L A Krasovskaya"
Article Synopsis
- Developing an efficient expression system for bacterial lytic enzymes is difficult because these enzymes target peptidoglycan in bacterial cell walls, which can lead to the lysis of the bacteria that produce them.
- The paper discusses the creation of an inducible expression system in Pseudomonas fluorescens Q2-87 for lytic peptidases AlpA and AlpB from Lysobacter sp. XL1, allowing for successful secretion of these proteins into the culture medium.
- The system uses a combination of a chromosomal endopeptidase gene under a T7lac promoter and a plasmid-borne phage T7 RNA polymerase, alongside optimized growth conditions to simplify the purification process, aiming to facilitate the
The N-terminal amino acid sequence (23 amino acid residues) and the amino acid composition of the extracellular bacteriolytic enzyme L1 of 21 kD from the bacterium Lysobacter sp. XL1 have been determined. The enzyme was hydrolyzed by trypsin, the resulting peptides were isolated, and their primary structures were determined.
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