Publications by authors named "L A Kolomiiets"
Background: Protein-based pharmaceuticals are among the fastest growing categories of therapeutic agents in the clinic and as commercial products, and typically target high-impact areas such as various cancers, autoimmune diseases and metabolic disorders. The aim of our work was to explore the possibility of reducing the level of aggregation and improve the stability of the recombinant proteins AIMP1/p43 (aminoacyl-tRNA synthetase complex component of the higher eukaryotes) and antitumor cytokine EMAP II (proteolytic cleavage product of AIMP1/p43) in combination with dextran-70 polysaccharide for structural-functional research and development of new sustainable biomedical products.
Methods: We studied interaction strength between these recombinant proteins with polymer by fluorescence spectroscopy and molecular docking.
The results of electron-microscopic investigation of the peculiarities of virus interrelations with cells of mesophyl of the potato leaves, which were infected with potato aucuba mosaic virus (PAMV) are presented. Aggregates of the virus particles have been revealed which are localized in cytoplasm between the cellular organoids and just near the cellular membrane. The significant destructural changes of the structure of chloroplasts and formations of virus-induced inclusions in them are observed.
View Article and Find Full Text PDFA study of placental detoxifying activity was undertaken using as the investigated indices glutathione transferase and glutathione reductase activities and total amount of SH-groups and those of low-molecular weight thiols. Malonic dialdehyde content was used as a measure of lipid peroxidation. The placental samples were obtained in several regions of the Ukraine judged either "clean", chemically polluted or radioactively contaminated.
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