Application of antioxidants in extender on bull sperm cryopreservation to reduce the male effect in dairy fertility.

Because male and female effects on fertility must be considered, it may be difficult to achieve accurate and repeatable fertility predictions using only sperm characteristics given differences in breed, health, and season. Improving sperm quality after cryopreservation may be a method to reduce the male effect on the fertility outcome. This study was conducted using 2 different Certified Semen Service approved extenders, one containing plant-derived antioxidants, to assess cryopreserved sperm quality and determine pregnancy per AI (P/AI) in a commercial dairy farm.

Spermatozoa cooled to 5°C one day after collection from porcine commercial semen doses retain sperm functionality with reduced bacterial load.

Background: Commercial porcine semen is stored at 17°C, leading to a reduction of sperm quality and increase of bacterial growth.

Objectives: To evaluate the effect of 5°C storage on porcine sperm functionality cooled one day after collection.

Materials And Methods: Semen doses (n = 40) were transported at 17°C and cooled at 5°C one day after collection.

Effect of season, genetic line and temperature during transport on sperm motility of commercial insemination doses of pooled boar semen: A retrospective study.

Commercial pooled semen from boars of maternal and terminal genetic lines was analysed over two consecutive years, as part of an external quality control program. Semen doses were prepared for two total sperm counts (2.0 × 10 /75 ml [n = 578] and 1.

A novel experimental design for boar sperm cryopreservation.

Cryopreservation of sperm is a routine technology in many livestock species, but not in swine. Frozen sperm must result in acceptable conception rates and produce 11 to 12 piglets/litter to be competitive with traditional cooled semen. The development of an extender that results in high post-thaw sperm quality and acceptable litter size requires the identification of factors that markedly affect post-thaw semen quality.

Validation of a new multiparametric protocol to assess viability, acrosome integrity and mitochondrial activity in cooled and frozen thawed boar spermatozoa.

Background: Motility, morphology, membrane integrity and DNA fragmentation are sperm characteristics routinely used to assess quality of boar spermatozoa. However, the evaluation of individual parameters has intrinsic restrictions in the estimation of potential fertility. Therefore, we aimed to validate a new multiparametric protocol to assess fertility potential through the evaluation of viability, acrosome integrity and mitochondrial activity within the same sperm population for cooled and frozen-thawed boar spermatozoa.