Publications by authors named "L A Everse"
Article Synopsis
- Major challenges in studying CD4+ effector cells and regulatory T cells (Tregs) arise from both cell types expressing CD25 and the necessity to analyze the forkhead box p3 marker in nonviable cells.
- The research highlights the utility of CD134 (OX40) combined with CD25 as a reliable method to distinguish live CD4+ effector cells from Tregs, particularly during adjuvant arthritis in rats.
- Findings indicate that CD134 and CD25 coexpression reliably identifies activated suppressive Tregs, while different lymph node locations show distinct functional variations in CD4+ T cell populations throughout the course of experimental arthritis.
T cells have an important role during the development of autoimmune diseases. In adjuvant arthritis, a model for rheumatoid arthritis, we found that the percentage of CD4+ T cells expressing the activation marker CD134 (OX40 antigen) was elevated before disease onset. Moreover, these CD134+ T cells showed a specific proliferative response to the disease-associated epitope of mycobacterial heat shock protein 60, indicating that this subset contains auto-aggressive T cells.
View Article and Find Full Text PDFWe examined which mechanism plays a dominant role in the rejection of solid SL2 lymphoma treated with locally applied IL-2 and/or IL-12. This treatment resulted in about 80% cures. There was a moderate influx of leukocytes in the tissue surrounding tumours; yet these cells failed to invade the solid tumours.
View Article and Find Full Text PDFCancer treatment with IL-2 and IL-12 is thought to work via enhancement of proliferation and activity of T cells and NK cells. Incubation of cytotoxic T lymphocytes (CTLs) and NK cells with IL-2 and/or IL-12 results in propagation of a distinct cell type called lymphokine-activated killers (LAK) characterized by increased lytic activity against many tumor types. Here we address the question whether cytokine therapy may be efficient in treatment of a LAK-insensitive tumor and, if so, which cell type, other than classic LAK cells, is responsible for tumor cell killing.
View Article and Find Full Text PDFThe effect of targeting strategies for improving the interaction of liposomal PorA with dendritic cells (DC) on the immunogenicity of PorA was investigated. PorA, a major antigen of Neisseria meningitidis, was purified and reconstituted in different types of (targeted) liposomes, i.e.
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