Publications by authors named "L A Elferink"
A first-year foundational science course grew beyond its scope. Revitalization of the course was driven by second and third-year medical students, who created new learning objectives and edited cases that were well-received by course facilitators and students. The students' role in the course revitalization is a novel approach.
View Article and Find Full Text PDFA non-synonymous single nucleotide polymorphism of the human serotonin 5-HT receptor (5-HTR) gene that converts a cysteine to a serine at amino acid codon 23 (Cys23Ser) appears to impact 5-HTR pharmacology at a cellular and systems level. We hypothesized that the Cys23Ser alters 5-HTR intracellular signaling via changes in subcellular localization in vitro. Using cell lines stably expressing the wild-type Cys23 or the Ser23 variant, we show that 5-HT evokes intracellular calcium release with decreased potency and peak response in the Ser23 versus the Cys23 cell lines.
View Article and Find Full Text PDFAcinar cells represent the primary target in necroinflammatory diseases of the pancreas, including pancreatitis. The signaling pathways guiding acinar cell repair and regeneration following injury remain poorly understood. The purpose of this study was to determine the importance of Hepatocyte Growth Factor Receptor/MET signaling as an intrinsic repair mechanism for acinar cells following acute damage and chronic alcohol-associated injury.
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- The MET receptor is crucial for development and organ renewal, but its dysregulation can lead to cancer progression, making it a valuable target for therapies.
- Developing high-throughput assays is essential for pinpointing regulators that can shutdown MET signaling.
- The described method employs high-throughput RNA interference screening alongside a receptor internalization test and In-Cell ELISA, allowing for efficient identification of factors influencing MET ubiquitination across various cell surface proteins.
Objectives: The hepatocyte growth factor receptor (Met) is frequently overexpressed in Head and Neck Squamous Cell Carcinoma (HNSCC), correlating positively with high-grade tumors and shortened patient survival. As such, Met may represent an important therapeutic target. The purpose of this study was to explore the role of Met signaling for HNSCC growth and locoregional dissemination.
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