Prognostic Role of Ultrasound Diagnostic Methods in Patients with Acute Decompensated Heart Failure.

Objectives: To evaluate the prognostic value (total mortality + repeated hospitalization for heart failure (HF)) of ultrasound diagnostic methods in patients with acute decompensated HF (ADHF).

Methods: The subjects were patients with chronic HF, who were hospitalized for ADHF. Using ultrasound methods-lung ultrasound, ultrasound assessment of hepatic venous congestion as per the venous excess ultrasound (VExUS) protocol, and indirect elastometry-we assessed the number of B-lines, hepatic venous congestion, and liver density of the patients.

Genetic stability of murine pluripotent and somatic hybrid cells may be affected by conditions of their cultivation.

Using mouse pluripotent teratocarcinoma PCC4azal cells and proliferating spleen lymphocytes we obtained a new type of hybrids, in which marker lymphocyte genes were suppressed, but expression the Oct-4 gene was not effected; the hybrid cells were able to differentiate to cardiomyocytes. In order to specify the environmental factors which may affect the genetic stability and other hybrid properties, we analyzed the total chromosome number and differentiation potencies of hybrids respectively to conditions of their cultivation. Particular attention was paid to the number and transcription activity of chromosomal nucleolus organizing regions (NORs), which harbor the most actively transcribed - ribosomal - genes.

[Problems of sex determination in birds exemplified by Gallus gallus domesticus].

The current views of sex determination in birds are considered mostly with the example of Gallus gallus domesticus, the species best studied in this respect. Data on the appearance of primordial germ cells, their migration to the primordial gonads, the role of hormonal factors in the regulation of sex differentiation, the sex chromosomes, putative genetic mechanisms of sex determination, and a possible contribution of dosage compensation are described. The review discusses the two best-grounded hypotheses on the roles of the Z and W chromosomes in sex determination.

[Mapping the chicken genome: problems and perspectives].

Various molecular methods are now used to map the chicken genome, including chromosome scraping, flow cytofluorimetry, zonal centrifugation, construction of chromosome-specific libraries, genetic analysis with polymorphic DNA markers, and in situ hybridization. Two main drawbacks are characteristic of existing maps of chicken chromosomes. First, classic genetic maps (i.

[Localization of various DNA sequences on the mitotic chromosomes of the chicken].

The chromosomal localization of the chicken transferrin receptor gene, as well as sequences that were homologous to the viral oncogene v-fos and the human gene families ZFY and SRY were determined by the method of nonisotopic DNA-DNA in situ hybridization. A correspondence was revealed between the Comptonian linkage group 10 and chromosome 1. A common origin of avian chromosome Z and mammalian chromosome Y is hypothesized.