Mapping the immune cell landscape of severe atopic dermatitis by single-cell RNA-seq.

Background: Efforts to profile atopic dermatitis (AD) tissues have intensified, yet comprehensive analysis of systemic immune landscapes in severe AD remains crucial.

Methods: Employing single-cell RNA sequencing, we analyzed over 300,000 peripheral blood mononuclear cells from 12 severe AD patients (Eczema area and severity index (EASI) > 21) and six healthy controls.

Results: Results revealed significant immune cell shifts in AD patients, including increased Th2 cell abundance, reduced NK cell clusters with compromised cytotoxicity, and correlated Type 2 innate lymphoid cell proportions with disease severity.

Stress behavior of FCC metallic thin films during thermal evaporation.

The development of stress in metallic thin films, monitored by in-situ curvature measurements during deposition, is analyzed. Three distinct stress regions including initial compressive, broad tensile, and incremental compressive stress were reported in terms of the film thickness (deposition time) by F. Spaepen.

Bicaval anastomosis reduces tricuspid regurgitation after heart transplantation.

The standard surgical technique utilizing two atrial cuff anastomoses has been used in the majority of transplant centers until recently when bicaval anastomoses was introduced. The purpose of this study was to compare the prevalence of tricuspid regurgitation after the bicaval and standard techniques of anastomosis. Heart transplantation was performed in 43 patients at our institution from April 1994 to December 2003: 15 by the standard technique (group A) and 28 by the bicaval technique (group B).