Publications by authors named "Kyung Wook Been"
Alopecia induced by aging or side effects of medications affects millions of people worldwide and impairs the quality of life; however, there is a limit to the current medications. Here, we identify a small transdermally deliverable 5-mer peptide (GLYYF; P5) that activates adiponectin receptor 1 (AdipoR1) and promotes hair growth. P5 sufficiently reproduces the biological effect of adiponectin protein via AMPK signaling pathway, increasing the expression of hair growth factors in the dermal papilla cells of human hair follicle.
View Article and Find Full Text PDFStructural characterization of a putative diguanylate cyclase conserved in hyperthermophiles.
Biochem Biophys Res Commun
October 2019
C-di-GMP, bis-(3'-5')-cyclic dimeric guanosine monophosphate, is a key signaling molecule that regulates many important physiological processes in bacteria. C-di-GMP is synthesized by diguanylate cyclase (DGC) containing the homodimeric GGDEF domain. There are many uncharacterized hypothetical proteins annotated as a putative DGC in bacteria including hyperthermophiles; however, their structures still remain unexplored.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates CRISPR Cpf1 endonucleases, which are enzymes that use single RNA guides to create specific double-stranded breaks in DNA, focusing on their targeting specificity compared to the widely used CRISPR-Cas9.
- - Researchers found that Cpf1 can handle mismatches in certain regions of the RNA guide, specifically tolerating them better in the 3' region compared to the 5' region.
- - A genome-wide analysis showed that Cpf1 has significantly fewer cleavage sites in the human genome than Cas9, and most off-target cuts by Cpf1 do not result in mutations when tested in cells, with the possibility of eliminating off-target effects through specific protein-RNA complexes