Which pathologic staining method can visualize the hyperacute infarction lesion identified by diffusion MRI?: A comparative experimental study.

Background: The study aimed to establish a staining method that could delineate the macroscopic lesion boundary of a hyperacute infarction depicted by diffusion-weighted MRI (DWI) and to validate the infarction boundary by comparing different staining methods.

New Method: Thirteen rats with 1 -h middle cerebral artery (MCA) infarction were included. Five different staining methods (Hematoxylin and eosin (H&E), Nissl, 2,3,5-triphenyltetrazolium hydrochloride (TTC), microtubule associated protein 2 (MAP2), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stains) were used to identify whether the hyperacute infarction could be histopathologically identified.

Retinopathy Induced by Zinc Oxide Nanoparticles in Rats Assessed by Micro-computed Tomography and Histopathology.

Nanotechnology has advanced at an extremely rapid pace over the past several years in numerous fields of research. However, the uptake of nanoparticles (NPs) into the body after administration through various routes may pose a risk to human health. In this study, we investigated the potential ocular toxicity of 20-nm, negatively- charged zinc oxide (ZnO) NPs in rats using micro-computed tomography (micro-CT) and histopathological assessment.

Expression of neighbor of Punc E11 in hepatocarcinogenesis induced by diethylnitrosamine.

To identify preneoplastic markers for hepatocarcino-genesis, the expression levels of neighbor of Punc E11 (Nope), E-cadherin and α-fetoprotein (AFP) were investigated in carcinogen-treated embryonic cell lineages. Mouse embryonic stem cells (ESCs), hepatic progenitor cells (HPCs), and hepatocyte‑like cells (HCs) representing 0, 22, and 40 days of liver differentiation, respectively, were treated in vitro with diethylnitrosamine (DEN) at 4 doses (0, 1, 5, and 15 mM) for 24 h. The expression of Nope, E-cadherin and AFP was examined by quantitative real-time PCR, western blotting and immunocytochemistry.

Ratio of angiopoietin-2 to angiopoietin-1 predicts mortality in acute lung injury induced by paraquat.

Background: To determine whether initial reactive oxygen species (ROS)-induced endothelial cell injury is involved in early death after paraquat intoxication and concentrations of angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), and von Willebrand factor (VWF) reflecting endothelial cell injury, we investigated the initial endothelial cell injury marker involved in the pathogenesis of death within 5 days after paraquat ingestion.

Material/methods: Sixty patients with paraquat poisoning were prospectively enrolled. Plasma samples were collected at admission.