Oxidative mechanisms of biological activity of low-intensity radiofrequency radiation.

This review aims to cover experimental data on oxidative effects of low-intensity radiofrequency radiation (RFR) in living cells. Analysis of the currently available peer-reviewed scientific literature reveals molecular effects induced by low-intensity RFR in living cells; this includes significant activation of key pathways generating reactive oxygen species (ROS), activation of peroxidation, oxidative damage of DNA and changes in the activity of antioxidant enzymes. It indicates that among 100 currently available peer-reviewed studies dealing with oxidative effects of low-intensity RFR, in general, 93 confirmed that RFR induces oxidative effects in biological systems.

Nonlinear regression models for determination of nicotinamide adenine dinucleotide content in human embryonic stem cells.

Recent evidence suggests that energy metabolism contributes to molecular mechanisms controlling stem cell identity. For example, human embryonic stem cells (hESCs) receive their metabolic energy mostly via glycolysis rather than mitochondrial oxidative phosphorylation. This suggests a connection of metabolic homeostasis to stemness.

N-(3-(4-Hydroxyphenyl)-propenoyl)-amino acid tryptamides as SIRT2 inhibitors.

A series of N-(3-(4-hydroxyphenyl)-propenoyl)-amino acid tryptamides was based on a previously reported new SIRT2 inhibitor from our group, and it was designed to study if the molecular size of the compound could be reduced. The most potent compounds, N-(3-(4-hydroxyphenyl)-propenoyl)-2-aminoisobutyric acid tryptamide and N-(3-(4-hydroxyphenyl)-propenoyl)-L-alanine tryptamide, were equipotent, 30% smaller in molecular weight, and slightly more selective (SIRT2/SIRT1) than the parent compound.

[Effect of omega-3 polyunsaturated fatty acids of animal origin on changes in regulation of lipid peroxidation in patients with ischemic heart disease].

The study's results showed reliable decrease in lipid peroxidation activity according to value of general sum of light, induced by hydrogen peroxide of initiated hemoluminescence and malonic dialdehyde concentration (P<0.05). The activity of antioxidant enzymes in the second group as compared with the control one has changed as follows: catalase concentration of blood serum of patients with ischemic heart disease increased by 59.

[Effect of modification in fatty acid composition of membrane phospholipids on reaction of isolated heart in acute ischemia-reperfusion].

The aim of this study was to evaluate the effects of a diet supplemented with omega-3 polyunsaturated fatty acids (eicosapentaenoic and docosahexaenoic) and tocopherol, which are the base of a preparation "Tekom", on a composition of myocardial phospholipid fatty acids, as well as on the metabolism of eicosanoids, free radical processes and the contractility of isolated working heart in rats at ischemia/reperfusion. Added to the diet within 4 weeks, "Tekom" induced an increase in the content of omega-3 polyunsaturated fatty acids in membranes of cardiomyocytes, a decrease in vasoactive metabolites of arachidonic acid and limitation of free radical processes. "Tekom" inhibited cardiac arrhythmias in the isolated working hearts of rats and improved the cardiac pump function at ischemia/reperfusion.

Differential regulation of the Sir2 histone deacetylase gene family by inhibitors of class I and II histone deacetylases.

The Sir2 histone deacetylase gene family consists of seven mammalian sirtuins (SIRTs) which are NAD-dependent histone/protein deacetylases. Sir2 proteins regulate, for instance, genome stability by chromatin silencing in yeast. In mammals, their function is still largely unknown.

Expression of transcriptional repressor proteins mSin3A and 3B during aging and replicative senescence.

Sin3 proteins have a key role in transcriptional repression mediated by histone deacetylation. Mammalian Sin3 proteins, mSin3A and 3B, act as adapter molecules which bind both to repressive transcription factors and to the methyl-CpG-binding proteins (MeCPs) and recruit histone deacetylases to assemble a multiprotein repressor complex. We have recently observed (Biochem.

[A comparative phylogenetic analysis of the coding portion of the genes for structural protein E1 in the Shi-Min' strain and in other strains of the classic hog cholera virus].

The primary nucleotide sequence of the fragment of E1 (gp51-55) gene (772 bp in length) of virulent strain Shi-Min' of classical swine fever virus (CSFV) has been determined. Multiple alignments of the E1 gene fragments of various strains and isolates of CSFV, which are homologues to the cloned fragment, has been carried out; the phylogenetic tree has been plotted and the consensus sequence has been determined. The strain Shi-Min', which was used as a control CSFV strain in former Soviet republics, and the referent strain Alfort were shown to be located on the opposite sides of the dendrogram, while Shi-Min' was more close to the western European group of CSFVs.