Publications by authors named "Kyoko Yamane"
Wasabi (Japanese horseradish, ) is the only cultivated species in the genus with functional components that provide a strong pungent flavor. To evaluate genetic resources for wasabi breeding, we surveyed variations in the two most abundant isothiocyanate (ITC) components in wasabi, allyl isothiocyanate (AITC) and 6-methylsulfinyl (hexyl) isothiocyanate (6-MSITC, hexaraphane). We also examined the phylogenetic relationships among 36 accessions of wild and cultivated wasabi in Japan using chloroplast DNA analysis.
View Article and Find Full Text PDFIn Japan, wasabi (Eutrema japonicum) is an important traditional condiment, and is recognized as an endemic species. In the present study, we generated a chromosome-level and haplotype-resolved reference genome for E. japonicum using PacBio CLR (continuous long reads), Illumina, and Hi-C sequencing data.
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- - The study sequenced the complete chloroplast genomes of seven wasabi and yuriwasabi accessions in Japan to investigate their phylogeny and evolutionary history, finding that these two species do not form a monophyletic group.
- - One yuriwasabi accession from Gifu was found to be closely related to E. yunnanense from China, indicating it may be a distinct species, with Japanese Eutrema species diverging from this lineage about 1.3 million years ago.
- - The research identified genetic differentiation among wasabi cultivars, leading to the development of genotyping markers based on polymorphic sites between specific cultivars, which will enhance understanding of their evolutionary relationships.
Microstructural changes such as insertions and deletions (=indels) are a major driving force in the evolution of non-coding DNA sequences. To better understand the mechanisms by which indel mutations arise, as well as the molecular evolution of non-coding regions, the number and pattern of indels and nucleotide substitutions were compared in the whole chloroplast genomes. Comparisons were made for a total of over 38 kb non-coding DNA sequences from 126 intergenic regions in two data sets representing species with different divergence times: sugarcane and maize and Oryza sativa var.
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- Colorectal cancer is a significant health concern, making prevention strategies essential, particularly through diet and nutrition, with garlic being noted for its medicinal properties.
- Aged garlic extract (AGE) was studied and shown to suppress the growth of colorectal cancer cells while having varying effects on their invasive capabilities, suggesting the influence of cancer cell type on garlic's effects.
- AGE not only inhibited the proliferation and tube formation of endothelial cells (important for blood vessel development) but also enhanced their adhesion to important structural proteins, indicating its potential as a chemopreventive agent against colorectal cancer by reducing angiogenesis.
This study analyzes intra- and interspecific variation in chloroplast DNA (cpDNA) in diploid Triticum-Aegilops species. This analysis focused on DNA sequence variation in noncoding regions of cpDNA, which included base-pair substitutions, insertion/deletions (indels, 50 loci pooled), microsatellites (7 loci pooled), and inversions. Nine of 13 Triticum-Aegilops species were successfully identified and genotyped using these data.
View Article and Find Full Text PDFWe investigated the phylogenetic and biogeographic relationships of natural populations of diploid and tetraploid Fagopyrum cymosum (Polygonaceae). Intraspecific variation of chloroplast DNA sequences was detected in three regions approximately 5 kb long in total: the 3' end of rbcL, accD and associated intergenic spacer region, the trnC (GCA)-rpoB spacer region, the trnK (UUU) intron, and the matK region. The accessions of F.
View Article and Find Full Text PDFTwo new Fagopyrum (Polygonaceae) species, F. gracilipedoides and F. jinshaense, are described.
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