Publications by authors named "Kyle V Murphy"

Article Synopsis
  • Polyhydroxyalkanoates (PHAs) are biodegradable polymers that serve as a carbon source for bacteria, but their breakdown products, such as β-hydroxybutyrate (BHB), lack well-characterized transport proteins.
  • The study identifies solute-binding proteins (SBPs) associated with ATP-binding cassette transporters that specifically recognize BHB, revealing potential for continuous monitoring of this important biomarker for various applications.
  • Through bioinformatics and experimental validation, a thermostable protein (Tt.2) from Thermus thermophilus showed the strongest binding affinity for BHB, supporting the hypothesis of these SBPs in bacterial metabolism and offering biotechnological opportunities.
View Article and Find Full Text PDF

While spine surgery has historically been performed in the inpatient setting, in recent years there has been growing interest in performing certain cervical and lumbar spine procedures on an outpatient basis. While conducting these procedures in the outpatient setting may be preferable for both the surgeon and the patient, appropriate patient selection is crucial. The employment of machine learning techniques for data analysis and outcome prediction has grown in recent years within spine surgery literature.

View Article and Find Full Text PDF

is an opportunistic human pathogen that can frequently be found at various body locations, such as the upper respiratory tract, nostrils, skin, and perineum. is responsible for causing a variety of conditions, which range from minor skin infections and food poisoning to life-threatening sepsis and endocarditis. Furthermore, has developed resistance to numerous antimicrobial agents, which has made treatment of infections difficult.

View Article and Find Full Text PDF

Purpose: To quantify cellular senescence in supraspinatus tendon and subacromial bursa of humans with rotator cuff tears and to investigate the in vitro efficacy of the senolytic dasatinib + quercetin (D+Q) to eliminate senescent cells and alter tenogenic differentiation.

Methods: Tissue was harvested from 41 patients (mean age, 62 years) undergoing arthroscopic rotator cuff repairs. In part 1 (n = 35), senescence was quantified using immunohistochemistry and gene expression for senescent cell markers (p16 and p21) and the senescence-associated secretory phenotype (SASP) (interleukin [IL] 6, IL-8, matrix metalloproteinase [MMP] 3, monocyte chemoattractant protein [MCP] 1).

View Article and Find Full Text PDF

Unsatisfactory failure rates following rotator cuff (RC) repair have led orthopaedic surgeons to explore biological augmentation of the healing enthesis. The subacromial bursa (SB) contains abundant connective tissue progenitor cells (CTPs) that may aid in this process. The purpose of the study was to investigate the influence of patient demographics and tear characteristics on the number of colony-forming units (CFUs) and nucleated cell count (NCC) of SB-derived CTPs.

View Article and Find Full Text PDF