The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in .

Promiscuous biotin ligases derived from the bacterial enzyme BirA are used to identify proteins vicinal to a bait protein, thereby defining its proxisome. Despite the popularity of this approach, surprisingly little is known about its use in prokaryotes. Here, we compared the activity of four widely used promiscuous biotin ligases in the cytoplasm of , a pathogenic subgroup of .

The T3SS of : Expression, Structure, Function, and Role in Vacuole Escape.

spp. are one of the leading causes of infectious diarrheal diseases. They are pathovars that are characterized by the harboring of a large plasmid that encodes most virulence genes, including a type III secretion system (T3SS).