The vectors went in two-by-two: Transduction efficiency and tolerability of dual and triple rAAV vector delivery following intravitreal injection for genome-editing applications.

Genome or prime editing has become a promising tool for the treatment of hereditary disorders affecting the inner retina, such as dominant optic neuropathies. In vivo delivery of gene editors, such as Cas9, is typically achieved using recombinant adeno-associated virus (rAAV) vectors, which have a broad range of cellular tropisms and are well tolerated following intravitreal administration. Owing to the large size of gene editing constructs and the limited carrying capacity of rAAV (<5.

Benchmarking DFT and Supervised Machine Learning: An Organic Semiconducting Polymer Investigation.

Using a training set consisting of twenty-two well-known semiconducting organic polymers, we studied the ability of a simple linear regression supervised machine learning algorithm to accurately predict the bandgap (BG) and ionization potential (IP) of new polymers. We show that using the PBE or PW91 exchange-correlation functionals and this simple linear regression, calculated BGs and IPs can be obtained with average percent errors of less than 3 and 4%, respectively. We then apply this method to predict the BG and IP of a group of new polymers composed of monomers used in the training set and their derivatives in AABB and ABAB orientations.

Mouse Homologue of Human HLA-DO Does Not Preempt Autoimmunity but Controls Murine Gammaherpesvirus MHV68.

H2-O (human HLA-DO) is a relatively conserved nonclassical MHC class II (MHCII)-like molecule. H2-O interaction with human HLA-DM edits the repertoire of peptides presented to TCRs by MHCII. It was long hypothesized that human HLA-DM inhibition by H2-O provides protection from autoimmunity by preventing binding of the high-affinity self-peptides to MHCII.

ATM supports gammaherpesvirus replication by attenuating type I interferon pathway.

Ataxia-Telangiectasia mutated (ATM) kinase participates in multiple networks, including DNA damage response, oxidative stress, and mitophagy. ATM also supports replication of diverse DNA and RNA viruses. Gammaherpesviruses are prevalent cancer-associated viruses that benefit from ATM expression during replication.