Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Preclinical Cardiotoxicity Screening in Cardio-Oncology.

• hiPSC-CM offer an alternative to in vivo models for predicting cardiotoxicity. • hiPSC-CM monolayers detect pro-arrhythmic effects; inotropic detection is less established. • Cardiac spheroids and engineered tissue may suit chronic cardiotoxicity studies (>2 weeks).

Charting the importance of filamin A posttranslational modifications.

Filamin A is an essential protein in the cell cytoskeleton because of its actin binding properties and unique homodimer rod-shaped structure, which organises actin into three-dimensional orthogonal networks imperative to cell motility, spreading and adhesion. Filamin A is subject to extensive posttranslational modification (PTM) which serves to co-ordinate cellular architecture and to modulate its large protein-protein interaction network which is key to the protein's role as a cellular signalling hub. Characterised PTMs include phosphorylation, irreversible cleavage, ubiquitin mediated degradation, hydroxylation and O-GlcNAcylation, with preliminary evidence of tyrosylation, carbonylation and acetylation.