Correction: Induction of Multiple miR-200/182 Members in the Brains of Mice Are Associated with Acute Herpes Simplex Virus 1 Encephalitis.

[This corrects the article DOI: 10.1371/journal.pone.

Induction of Multiple miR-200/182 Members in the Brains of Mice Are Associated with Acute Herpes Simplex Virus 1 Encephalitis.

Important roles of microRNAs (miRNAs) in regulating the host response during viral infection have begun to be defined. However, little is known about the functional roles of miRNAs within an in vivo acute viral encephalitis model. We therefore identified global changes in miRNA expression during acute herpes simplex virus type 1 (HSV-1) encephalitis (HSVE) in mice.

A user-friendly computational workflow for the analysis of microRNA deep sequencing data.

Second-generation high-throughput sequencing is a robust and inexpensive methodology that is becoming an increasingly common technique for the study of microRNA (miRNA) expression levels in the central nervous system. This method allows for the identification of both known and novel miRNAs, reporting on the qualitative and quantitative levels these RNA species represent in any given sample. Numerous bioinformatic programs are currently available to analyze deep sequencing data but many require at least a partial understanding of the command line interface.