A novel enzyme-linked immunosorbent assay system for the quantitative analysis of Carassius auratus vitellogenin.

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantitatively detect Carassius auratus vitellogenin (VTG) levels. The protein levels in fish plasma are useful aquatic biomarkers of estrogenic compounds. This procedure involved an ELISA using monoclonal antibodies of CVmA2 and CVmA7 against Carassius auratus VTG, and CVmA7 conjugated to horseradish peroxidase as the detection antibody.

Problems facing Korean hospitals and possible countermeasures.

Korea has a unique health care system, of which the private sector comprises most of the country's health resources: 88% of the beds and 91% of specialists in Korea, but are funded by public financing, such as national health insurance and the national aid program. However, the public financing pays only 50% of actual costs and the patient's co-payment is still high. Healthcare organizations in Korea are categorized into four types; tertiary care hospitals, general hospitals, hospitals and clinics by scale of operator: number of beds.