[Accumulation of heavy metals in cereal crops and ways of reducing their levels].

The study deals with the levels of heavy metals in the raw grain grown in the agricultural lands of the Oryol Region and provides the results of a trial of a complex enzymatic agent based on phitase and succinic acid used to reduce the levels of heavy levels in the wheat and rye grains during manufacture of grain bread. The use of the complex-acting enzymatic agent based on phitase obtained from the fungus Penicillium canescens in combination with 0.1% succinic acid of the mass of wheat and rye grains when the latter is prepared for the manufacture of grain bread has been established to reduce the concentrations of nickel, lead, cadmium, and chromium, by lowering the levels of MAFAM, spore-forming bacteria, moulds, and yeasts in the raw materials.

[Analysis of the correlation between genetic and immunohistochemical markers (RB1, p16 and p53 gene regultors of a cell cycle) in sporadic mammary gland carcinoma].

The results of an investigation of the RB 1, p16 and p53 genes in sporadic breast cancer through molecular and immunohistochemical studies are presented.

[Regulatory genes of garden pea (Pisum sativum L.) controlling the development of nitrogen-fixing nodules and arbuscular mycorrhiza: a review of basic and applied aspects].

The review sums up the long experience of the authors and other researchers in studying the genetic system of garden pea (Pisum sativum L.), which controls sthe development of nitrogen-fixing symbiosis and arbuscular mycorrhiza. A justified phenotypic classification of pea mutants is presented.

Methylation of the BIN1 gene promoter CpG island associated with breast and prostate cancer.

Background: Loss of BIN1 tumor suppressor expression is abundant in human cancer and its frequency exceeds that of genetic alterations, suggesting the role of epigenetic regulators (DNA methylation). BIN1 re-expression in the DU145 prostate cancer cell line after 5-aza-2'-deoxycytidine treatment was recently reported but no methylation of the BIN1 promoter CpG island was found in DU145.

Methods: Methylation-sensitive arbitrarily-primed PCR was used to detect genomic loci abnormally methylated in breast cancer.

[Algorithm of therapy in neonates with developmental malformations depending on causes and severity of illness in the intensive care unit].

The results of treatment for congenital malformations requiring emergency interventions depend on the timeliness and correctness of diagnosis of a defect, on the performance of resuscitative and surgical interventions, the correct evaluation of a neonate's condition at different stages of treatment. The score rating of the patient's condition is objective and may be an evidence-based medicine tool. Prior to the emergence of the universal scale, many authors used their own developments.

Effect of 5HT2C-receptor agonist MK-212 on blood corticosterone level and behavior in mice.

A dose-dependent the effect of 5HT2C-receptor agonist MK-212 on mouse behavior was demonstrated. Intraperitoneal injection of MK-212 in high doses (0.5 and 1.

Effect of 5-HT2C receptor antagonist RS 102221 on mouse behavior.

Injection of RS 102221 (selective antagonist of serotonin 5-HT2C receptors) in a dose of 2 mg/kg reduced anxiety of mice in the light-darkness test and decreased the amplitude of the startle reflex. RS 102221 in a dose of 1 mg/kg reduced prestimulus inhibition of the startle reflex. No behavioral changes in Porsolt test and motor activity in the open field test were detected.

[Electric impulse therapy of hypertensive patients].

Patients with mild arterial hypertension and hypersympathicotonia treated with electroimpulse therapy improved their clinicofunctional state, blood pressure (especially systolic pressure), its variability, sympathic activity, central regulation of cardiac rhythm, capillary reserves.

[The effects of neonatal saline injections on behaviours in DBA/2 male mice].

DBA/2 male mice were exposed to the injections of the saline (0.01 ml/g i.p.

Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family.

Haloacid dehalogenase (HAD)-like hydrolases are a vast superfamily of largely uncharacterized enzymes, with a few members shown to possess phosphatase, beta-phosphoglucomutase, phosphonatase, and dehalogenase activities. Using a representative set of 80 phosphorylated substrates, we characterized the substrate specificities of 23 soluble HADs encoded in the Escherichia coli genome. We identified small molecule phosphatase activity in 21 HADs and beta-phosphoglucomutase activity in one protein.

[The effect of neonatal administration of monosodium glutamate on behavior and blood corticosterone level].

DBA/2 male mice were treated with monosodium glutamate (MSG) in a dose of 4 mg/g on 1, 3, 5, 7, 9 days after birth. Saline treated and intact males were used as control groups. MSG treated males displayed decreased number of crossed squares, rearings, entries in the centre and time in the centre of open field in comparison with saline-treated but not intact animals.

[Characteristics of associated microflora of ejaculate from males with gonococcal infections].

Species composition and biological properties of aerobic and anaerobic microflora from an ejaculate from males with acute and chronic gonococcal infection and from healthy men were studied. Patients with gonococcal infection were shown to present quantitative and qualitative changes in microbiocenosis of the reproductive tract. Patients with acute gonorrhea displayed decreased species diversity in contrast to healthy persons, while different species of associated with gonococci microbes with high persistent potential, were cultured in patients suffering from a chronic form of infection.

[Proteins associated with mitochondrial DNA protect it against the action of X-rays and hydrogen peroxide].

It has been suggested in a number of investigations that the high vulnerability of mitochondrial DNA to reactive oxygen species and other damaging agents is due to the absence in mitochondria of histones complexed with DNA. In the present study it was shown that DNA-binding proteins of mitochondrial nucleoids were able to shield mitochondrial DNA from X-ray radiation and hydrogen peroxide, as nuclear histones did. Mitochondria, mitochondrial nucleoid proteins, and histones were isolated from mouse liver cells.

[The stress effect in the prenatal period on sexual excitation and sexual orientation of the mice males].

Estrus female behind holed transparent partition produced sexual motivation and sexual arousal in males. It was manifested in behavioral changes (an increase in time spent near the partition) and the testosterone level augmentation in blood. Female mice were exposed to stress (1 h/day restraint) in the last week of gestation.

[The individual differences in responses of cancer patient blood cells on radiation treatment during the chemotherapy].

A comparative comet-assay study of X-ray influence on DNA of leukocytes of peripheral blood from both cancer patients in the course of chemotherapy and on healthy donors was carried out. The amount of DNA registered in comet tails of blood samples from 18 healthy donors was between 0.8-3.

[Study of DNA-binding proteins of rat liver mitochondria].

Acid-soluble proteins able to form DNA-protein complexes in the presence of physiological concentration of NaCl were isolated from rat liver mitochondria. Electrophoretic analysis of these proteins in 15% polyacrylamide gel showed that mitochondrial acid-soluble proteins include of approximately 20 polypeptides with molecular weight of 10-120 kDa. The fraction of acid-soluble proteins can be separated into basic and acidic proteins by chromatography on DEAE cellulose.

[Clinical polymorphism of autoimmune polyglandular syndrome of type 1. Role of molecular genetic diagnosis].

Autoimmune polyglandular syndrome type 1 (APS-1) is a rare monogenous disease with autosomal-recessive inheritance. The gene responsible for the development of this disease is designated as the gene AIRE and encodes for the protein autoimmune regulator whose function is unknown. The disease more frequently manifests itself in childhood.

Effect of neonatal injection of sodium glutamate and diethylnitrosamine on hepatocarcinogenesis, reproductive and adrenocortical systems of male mice.

Neonatal injection of sodium glutamate before injection of diethylnitrosamine decreased the number of tumor nodes in the liver of male mice, decreased the weight of the testes and adrenals and blood level of testosterone (but increased blood level of corticosterone), impaired recovery of diethylnitrosamine-disturbed sexual motivation in half of males. Anticarcinogenic effect of sodium glutamate is explained by feminization of males under its effect.

[Sodium glutamate on some physiological features and chemically induced hepatocarcinogenesis in neontal period in male mice].

A single injection of diethylnitrosamine 50 mg/kg to 12-day old CBA mice led to development of 50.7 +/- 4.8 liver tumor nodules in males and 3.

DNA-binding proteins of mammalian mitochondria.

Acid-soluble proteins were isolated from liver and spleen mitochondria and their ability to form complexes with DNA was investigated. According to electrophoresis data, acid-soluble proteins include about 20 polypeptides ranging in the molecular mass from 10 to 120 kDa. It was found that acid-soluble proteins form stable DNA-protein complexes at a physiological NaCl concentration.

[Study of DNA-binding proteins in mitochondria of rat liver gamma-irradiation].

Acid-soluble proteins were isolated from the liver mitochondria of control and irradiated (8 Gy) rats. By means of electrophoresis in 15% polyacrylamide gel, these proteins were separated into more than 20 polypeptides of molecular masses between 10 and 120 kDa. The irradiation of rats with a dose of 8 Gy led to changes in the polypeptide content of mitochondrial acid-soluble proteins in the postradiation period.

Enzyme genomics: Application of general enzymatic screens to discover new enzymes.

In all sequenced genomes, a large fraction of predicted genes encodes proteins of unknown biochemical function and up to 15% of the genes with "known" function are mis-annotated. Several global approaches are routinely employed to predict function, including sophisticated sequence analysis, gene expression, protein interaction, and protein structure. In the first coupling of genomics and enzymology, Phizicky and colleagues undertook a screen for specific enzymes using large pools of partially purified proteins and specific enzymatic assays.

A high throughput method for the detection of metalloproteins on a microgram scale.

Proteins that bind transition metals make up a substantial portion of the proteome, and the identification of a metal cofactor in a protein can greatly facilitate its functional assignment and help place it in the context of known cellular pathways. Existing methods for the detection of metalloproteins generally consume large amounts of protein, require expensive equipment, or are very labor intensive, rendering them unsuitable for use in high throughput proteomic initiatives. Here we present a method for the identification of metalloproteins that contain iron, copper, manganese, cobalt, nickel, and/or zinc that is sensitive, quick, robust, inexpensive, and can be performed with standard laboratory equipment.

[Immunoblot for verifying the results of screening of antibodies to hepatitis C virus in blood donors].

A conclusion is made, on the basis of independent research results and published data, on the need to verify, compulsorily, the screening finding of donor blood for hepatitis virus by immunoblot and, in extra complicated cases, by PCR.