High-Throughput Evaluation of Natural Diversity of F-Type ATP Synthase Rotor Ring Stoichiometries.

Adenosine triphosphate (ATP) synthases are large enzymes present in every living cell. They consist of a transmembrane and a soluble domain, each comprising multiple subunits. The transmembrane part contains an oligomeric rotor ring (c-ring), whose stoichiometry defines the ratio between the number of synthesized ATP molecules and the number of ions transported through the membrane.

[Study of the pharmacokinetics of extended release sildenafil].

Purpose Of The Study: Comparison of the pharmacokinetic characteristics of the drug Vildegra registered in the Russian Federation with literature data for the original drug Viagra.

Materials And Methods: Study design: prospective, open-label in healthy volunteers with a single oral dose on an empty stomach. The study included 48 male volunteers aged 18 to 45 years with a verified diagnosis of "healthy.

Mechanical, thermal, and water absorption properties of HDPE/barley straw composites incorporating waste rubber.

This study investigates the mechanical, thermal, and water absorption properties of high-density polyethylene (HDPE) composites filled with barley straw and varying amounts of waste rubber. The research aims to develop sustainable materials that repurpose agricultural and industrial waste while addressing resource scarcity and waste management challenges. Composites were prepared using a twin-rotor mixer and hydraulic press, with waste rubber content varying from 0 to 20 wt%.

Unlocking nature's brilliance: using Antarctic extremophile Shewanella baltica to biosynthesize lanthanide-containing nanoparticles with optical up-conversion.

Both lanthanide-containing and fluorine-containing nanomaterials present challenging targets for microbial biosynthesis because these elements are toxic to most bacteria. Here, we overcome these challenges by using an Antarctic Shewanella baltica strain that tolerates these elements and report the first biosynthesis of lanthanide-doped fluoride nanoparticles (NPs) from them. NaYF NPs doped with Er/Yb are prototypical lanthanide-based upconverting nanoparticles (UCNPs) with upconverted luminescence at visible wavelengths under infrared excitation.

Search for Rare b→dℓ^{+}ℓ^{-} Transitions at Belle.

We present the results of a search for the b→dℓ^{+}ℓ^{-} flavor-changing neutral-current rare decays B^{+,0}→(η,ω,π^{+,0},ρ^{+,0})e^{+}e^{-} and B^{+,0}→(η,ω,π^{0},ρ^{+})μ^{+}μ^{-} using a 711  fb^{-1} data sample that contains 772×10^{6}  BB[over ¯] events. The data were collected at the ϒ(4S) resonance with the Belle detector at the KEKB asymmetric-energy e^{+}e^{-} collider. We find no evidence for signal and set upper limits on branching fractions at the 90% confidence level in the range (3.

Binuclear Coordination Assemblies of Metal Tetraphenylporphyrins (M = Mn, Fe, and In) with Anionic Thioindigo Bridges as Promising Magnetic Systems with Tunable Properties.

A series of hybrids comprising two metal (Mn, Fe, and In) tetraphenylporphyrins axially substituted with anionic bidentate -thioindigo ligands (TI) were obtained. Substitution of the axial chloride anion by an oxygen atom of the dye forms short M-O bonds. Crystalline binuclear assemblies (TI)·{[MnTPP]·[MnTPP]}·CHCl ( = 2 for or 1 for ) and (TI){[MTPP]}·CHCl (M = Fe and = 2 for , M = In and = 1 for ) were synthesized.

Microbial green synthesis of luminescent terbium sulfide nanoparticles using E. Coli: a rare earth element detoxification mechanism.

Background: Rare-earth sulfide nanoparticles (NPs) could harness the optical and magnetic features of rare-earth ions for applications in nanotechnology. However, reports of their synthesis are scarce and typically require high temperatures and long synthesis times.

Results: Here we present a biosynthesis of terbium sulfide (TbS) NPs using microorganisms, identifying conditions that allow Escherichia coli to extracellularly produce TbS NPs in aqueous media at 37 °C by controlling cellular sulfur metabolism to produce a high concentration of sulfide ions.

Isolation and Characterization of Salmonella Bacteriophages as Potential Agents for Phage Therapy of Antibiotic-Resistant Intestinal Infections.

Two bacteriophages specifically active against to pathogenic strains of the Salmonella genus were isolated. The morphology of phage colonies (size, transparency, and shape of the plaque edge, and halo) and the spectrum of their lytic activity and interaction with microbial cells (adsorption rate, duration of the latency, and reproductive efficiency) were examined. Using genome-wide sequencing, we determined the taxonomic position of bacteriophages and verified the absence of unwanted genes encoding toxins, adhesins, and invasins, as well as pathogenicity islands responsible for antibiotic resistance.

[The history of the institute of higher and additional professional education of the Endocrinology Reserch Centre].

The Endocrinology Reserch Centre is proud not only of its achievements in the area of personalized approach to the examination and treatment of patients in accordance with the principles of evidence-based medicine, the use of modern diagnostic and treatment technologies, but also of its rich history of training scientific and medical personnel. For many years the Center has been attracting the best and most talented graduates of higher medical institutions, becoming the "alma mater" for young doctors - endocrinologists, pediatric endocrinologists and nutritionists. Specialists, graduates of NMRC, make a significant contribution to the development of medicine, conducting research, creating innovative methods of treatment and helping patients not only in our country, but also abroad.

Structure of Polaronic Centers in Proton-Intercalated WO Scheelite-Type Tungstates.

The studies of polaronic centers in a homologous series of scheelite-type compounds WO ( = Ca, Sr, Ba) were performed using the W L-edge and Sr K-edge X-ray absorption spectroscopy combined with the reverse Monte Carlo simulations, X-ray photoelectron spectroscopy (XPS), and first-principles calculations. Protonated scheelites HWO were produced using acid electrolytes in a one-step route at ambient conditions. The underlying mechanism behind this phenomenon can be ascribed to the intercalation of H into the crystal structure of tungstate, effectively resulting in the reduction of W to W, i.

Evaluation of pulse-jet baghouse dust collectors' contribution to CO emissions.

Dust cleaning systems are mandatory for use almost in any manufacturing process. Their market size is expected at US$10.77 billion by 2030 growing from US$7.

The Development and Atomic Structure of Zinc Oxide Crystals Grown within Polymers from Vapor Phase Precursors.

Sequential infiltration synthesis (SIS), also known as vapor phase infiltration (VPI), is a quickly expanding technique that allows growth of inorganic materials within polymers from vapor phase precursors. With an increasing materials library, which encompasses numerous organometallic precursors and polymer chemistries, and an expanding application space, the importance of understanding the mechanisms that govern SIS growth is ever increasing. In this work, we studied the growth of polycrystalline ZnO clusters and particles in three representative polymers: poly(methyl methacrylate), SU-8, and polymethacrolein using vapor phase diethyl zinc and water.

Measurement of the Pion Form Factor with CMD-3 Detector and Its Implication to the Hadronic Contribution to Muon (g-2).

The cross section of the process e^{+}e^{-}→π^{+}π^{-} has been measured in the center-of-mass energy range from 0.32 to 1.2 GeV with the CMD-3 detector at the electron-positron collider VEPP-2000.

The limit to evolutionary rescue depends on ploidy in yeast exposed to nystatin.

The number of copies of each chromosome, or ploidy, of an organism is a major genomic factor affecting adaptation. We set out to determine how ploidy can impact the outcome of evolution, as well as the likelihood of evolutionary rescue, using short-term experiments with yeast () in a high concentration of the fungicide nystatin. In similar experiments using haploid yeast, the genetic changes underlying evolutionary rescue were highly repeatable, with all rescued lines containing a single mutation in the ergosterol biosynthetic pathway.

First Measurement of R(X_{τ/ℓ}) as an Inclusive Test of the b→cτν Anomaly.

We measure the tau-to-light-lepton ratio of inclusive B-meson branching fractions R(X_{τ/ℓ})≡B(B→Xτν)/B(B→Xℓν), where ℓ indicates an electron or muon, and thereby test the universality of charged-current weak interactions. We select events that have one fully reconstructed B meson and a charged lepton candidate from 189  fb^{-1} of electron-positron collision data collected with the Belle II detector. We find R(X_{τ/ℓ})=0.

Reengineering of a flavin-binding fluorescent protein using ProteinMPNN.

Recent advances in machine learning techniques have led to development of a number of protein design and engineering approaches. One of them, ProteinMPNN, predicts an amino acid sequence that would fold and match user-defined backbone structure. Its performance was previously tested for proteins composed of standard amino acids, as well as for peptide- and protein-binding proteins.

Effect of chitosan-epoxy ratio in bio-based adhesive on physical and mechanical properties of medium density fiberboards from mixed hardwood fibers.

Chitosan and bio-based epoxy resins have emerged as promising formaldehyde-free replacements for traditional urea-formaldehyde (UF) adhesives in engineered wood products. This study evaluated five chitosan-to-epoxy weight ratios (3:1, 2:1, 1:1, 1:2, 1:3) as adhesives for hot-pressing medium density fiberboards (MDF) using mixed hardwood fibers. Increasing the epoxy ratio reduced viscosity and gel time, facilitating spraying and fast curing.

Testing of Polymer Composites for Manufacturing of Sprayer Nozzles.

Wear is the leading cause of nozzle failure. The durability of the nozzle is affected by the material it is made from. Traditional materials are ceramics, stainless steel, brass, and polymers.

Overlay databank unlocks data-driven analyses of biomolecules for all.

Tools based on artificial intelligence (AI) are currently revolutionising many fields, yet their applications are often limited by the lack of suitable training data in programmatically accessible format. Here we propose an effective solution to make data scattered in various locations and formats accessible for data-driven and machine learning applications using the overlay databank format. To demonstrate the practical relevance of such approach, we present the NMRlipids Databank-a community-driven, open-for-all database featuring programmatic access to quality-evaluated atom-resolution molecular dynamics simulations of cellular membranes.

Extracellular vesicles from activated platelets possess a phospholipid-rich biomolecular profile and enhance prothrombinase activity.

Background: Extracellular vesicles (EVs), in particular those derived from activated platelets, are associated with a risk of future venous thromboembolism.

Objectives: To study the biomolecular profile and function characteristics of EVs from control (unstimulated) and activated platelets.

Methods: Biomolecular profiling of single or very few (1-4) platelet-EVs (control/stimulated) was performed by Raman tweezers microspectroscopy.

Effect of High-Pressure Torsion on Phase Formation and Mechanical Properties of a High-Entropy TiZrHfMoCrCo Alloy.

This investigation delved into the alterations in the mechanical properties of a TiZrHfMoCrCo high-entropy alloy due to phase transformations induced by high-pressure torsion (HPT). The alloy's genesis involved levitation melting within an argon atmosphere, presenting two distinct states for analysis: the initial, post-manufacturing state and the state subsequent to HPT treatment. The original alloy featured a composition comprising a singular A2 phase with a bcc lattice and two Laves phases, C15 and C14.